{"id":99415,"date":"2026-09-02T15:24:50","date_gmt":"2026-09-02T07:24:50","guid":{"rendered":"https:\/\/www.wsisp.com\/helps\/99415.html"},"modified":"2026-09-02T15:24:50","modified_gmt":"2026-09-02T07:24:50","slug":"%e6%97%a0%e5%8a%a8%e7%89%a9%e6%ba%90%e9%87%8d%e7%bb%84%e4%ba%batgf-%ce%b21%e5%ae%9e%e6%93%8d%e8%a6%81%e7%82%b9%e4%b8%8e%e7%a7%91%e7%a0%94%e9%80%89%e5%9e%8b%e9%81%bf%e5%9d%91%e6%8c%87%e5%8d%97","status":"publish","type":"post","link":"https:\/\/www.wsisp.com\/helps\/99415.html","title":{"rendered":"\u65e0\u52a8\u7269\u6e90\u91cd\u7ec4\u4ebaTGF\u2011\u03b21\u5b9e\u64cd\u8981\u70b9\u4e0e\u79d1\u7814\u9009\u578b\u907f\u5751\u6307\u5357"},"content":{"rendered":"<p>\u91cd\u7ec4\u4eba\u8f6c\u5316\u751f\u957f\u56e0\u5b50\u03b21&#xff08;\u65e0\u52a8\u7269\u6e90&#xff09;\u6d3b\u6027\u7075\u654f&#xff0c;\u86cb\u767d\u5b58\u5728\u6f5c\u4f0f\u590d\u5408\u7269\u7ed3\u6784&#xff0c;\u590d\u6eb6\u3001\u6fc0\u6d3b\u3001\u5206\u88c5\u50a8\u5b58\u64cd\u4f5c\u90fd\u4f1a\u76f4\u63a5\u5f71\u54cd\u6700\u7ec8\u5b9e\u9a8c\u7ed3\u679c\u3002\u5f88\u591a\u79d1\u7814\u4eba\u5458\u62ff\u5230\u9ad8\u6307\u6807\u7684 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0.1\u20111ng\/mL&#xff1b;\u514d\u75ab\u7ec6\u80de\u5206\u5316\u5b9e\u9a8c\u9700\u8981\u6839\u636e\u7ec6\u80de\u7c7b\u578b\u8bbe\u7f6e\u68af\u5ea6\u3002 \u5b9e\u9a8c\u5fc5\u987b\u8bbe\u7f6e\u5b8c\u6574\u5bf9\u7167\u7ec4&#xff1a;\u6eb6\u5242\u5bf9\u7167\u7ec4\u3001\u901a\u8def\u6291\u5236\u5242\u5bf9\u7167\u7ec4&#xff08;\u4f8b\u5982 SB431542&#xff09;&#xff0c;\u5f53\u52a0\u5165\u6291\u5236\u5242\u4e4b\u540e\u7ec6\u80de\u8868\u578b\u5f97\u5230\u660e\u663e\u9006\u8f6c&#xff0c;\u624d\u80fd\u591f\u8bc1\u660e\u89c2\u6d4b\u73b0\u8c61\u7531 TGF\u2011\u03b21\u2011Smad \u901a\u8def\u4ecb\u5bfc&#xff0c;\u6392\u9664\u5185\u6bd2\u7d20\u3001\u6742\u86cb\u767d\u5e26\u6765\u7684\u975e\u7279\u5f02\u6027\u5e72\u6270\u3002<\/p>\n<h3>\u4e09\u3001\u65e0\u52a8\u7269\u6e90 TGF\u2011\u03b21 \u79d1\u7814\u9009\u578b\u6838\u5fc3\u6807\u51c6<\/h3>\n<p>\u7b2c\u4e00&#xff0c;\u786e\u8ba4\u8868\u8fbe\u5bbf\u4e3b\u3002HEK293 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\u7b2c\u4e09&#xff0c;\u6838\u67e5\u5b8c\u6574\u8d28\u63a7\u6587\u4ef6&#xff0c;\u9664 SDS\u2011PAGE \u7eaf\u5ea6\u5916&#xff0c;\u91cd\u70b9\u67e5\u770b\u5185\u6bd2\u7d20\u6307\u6807\u4ee5\u53ca\u57fa\u4e8e TF\u20111 \u7ec6\u80de\u6291\u5236\u589e\u6b96\u7684\u751f\u7269\u6d3b\u6027\u68c0\u6d4b\u6570\u636e&#xff0c;\u5355\u7eaf\u7535\u6cf3\u7eaf\u5ea6\u4e0d\u80fd\u4ee3\u8868\u86cb\u767d\u771f\u5b9e\u751f\u7269\u6d3b\u6027\u3002<\/p>\n<h3>\u56db\u3001\u9ad8\u9891\u79d1\u7814\u8bef\u533a\u68b3\u7406<\/h3>\n<p>\u8bef\u533a\u4e00&#xff1a;\u76f4\u63a5\u7528 PBS \u590d\u6eb6\u51bb\u5e72 TGF\u2011\u03b21\u3002\u8be5\u86cb\u767d\u5728\u4e2d\u6027 PBS \u4e2d\u6eb6\u89e3\u6027\u8f83\u5dee&#xff0c;\u4f1a\u9020\u6210\u5b9e\u9645\u6709\u6548\u6d53\u5ea6\u8fdc\u4f4e\u4e8e\u6807\u79f0\u503c&#xff0c;\u5e94\u5f53\u9075\u5faa\u8bf4\u660e\u4e66\u4f7f\u7528\u9178\u6027\u6eb6\u5242\u5b8c\u6210\u521d\u59cb\u590d\u6eb6\u3002 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