{"id":94447,"date":"2026-08-14T14:24:50","date_gmt":"2026-08-14T06:24:50","guid":{"rendered":"https:\/\/www.wsisp.com\/helps\/94447.html"},"modified":"2026-08-14T14:24:50","modified_gmt":"2026-08-14T06:24:50","slug":"%e5%9c%9f%e5%a3%a4%e4%ba%ae%e6%b0%a8%e9%85%b8%e6%b0%a8%e5%9f%ba%e8%82%bd%e9%85%b6%ef%bc%88s-lap%ef%bc%89%e6%b4%bb%e6%80%a7%e6%a3%80%e6%b5%8b%e6%8c%87%e5%8d%97%ef%bc%9a%e6%b0%ae%e5%be%aa%e7%8e%af","status":"publish","type":"post","link":"https:\/\/www.wsisp.com\/helps\/94447.html","title":{"rendered":"\u571f\u58e4\u4eae\u6c28\u9178\u6c28\u57fa\u80bd\u9176\uff08S-LAP\uff09\u6d3b\u6027\u68c0\u6d4b\u6307\u5357\uff1a\u6c2e\u5faa\u73af\u673a\u5236\u3001\u5fae\u91cf\u5b9e\u64cd\u4e0e\u9009\u8d2d\u89e3\u6790"},"content":{"rendered":"

\u5728\u571f\u58e4\u751f\u6001\u5b66\u3001\u519c\u7530\u5730\u529b\u6f14\u53d8\u53ca\u5fae\u751f\u7269\u529f\u80fd\u591a\u6837\u6027\u7814\u7a76\u4e2d，\u571f\u58e4\u6c2e\u7d20\u7684\u77ff\u5316\u4e0e\u6709\u6548\u6027\u662f\u51b3\u5b9a\u690d\u7269\u751f\u957f\u548c\u751f\u6001\u7cfb\u7edf\u751f\u4ea7\u529b\u7684\u6838\u5fc3\u56e0\u7d20\u3002\u571f\u58e4\u4e2d\u7684\u7edd\u5927\u90e8\u5206\u6c2e\u7d20\u4ee5\u6709\u673a\u6c2e（\u5982\u86cb\u767d\u8d28\u3001\u591a\u80bd\u53ca\u6c28\u57fa\u7cd6）\u7684\u5f62\u5f0f\u5b58\u5728，\u5fc5\u987b\u7ecf\u8fc7\u571f\u58e4\u80de\u5916\u9176\u7684\u964d\u89e3\u624d\u80fd\u8f6c\u5316\u4e3a\u690d\u7269\u548c\u5fae\u751f\u7269\u53ef\u5229\u7528\u7684\u65e0\u673a\u6c2e\u6216\u5c0f\u5206\u5b50\u6c28\u57fa\u9178\u3002\u5176\u4e2d，\u571f\u58e4\u4eae\u6c28\u9178\u6c28\u57fa\u80bd\u9176（Soil Leucine Aminopeptidase，\u7b80\u79f0 S-LAP） \u662f\u4e00\u79cd\u5173\u952e\u7684\u80bd\u94fe\u5185\u5207\/\u5916\u5207\u6c34\u89e3\u9176\u3002\u5b83\u4e13\u4e00\u6027\u5730\u50ac\u5316\u591a\u80bd\u548c\u86cb\u767d\u8d28 N-\u672b\u7aef\u7684\u4eae\u6c28\u9178\u53ca\u5176\u4ed6\u758f\u6c34\u6027\u6c28\u57fa\u9178\u6b8b\u57fa\u6c34\u89e3，\u91ca\u653e\u6e38\u79bb\u6c28\u57fa\u9178\u3002S-LAP \u6d3b\u6027\u4e0d\u4ec5\u76f4\u63a5\u53cd\u6620\u4e86\u571f\u58e4\u5fae\u751f\u7269\u5bf9\u6709\u673a\u6c2e\u7684\u5206\u89e3\u80fd\u529b\u548c\u6c2e\u7d20\u9700\u6c42，\u4e5f\u662f\u8bc4\u4f30\u571f\u58e4\u80a5\u529b\u3001\u73af\u5883\u6c61\u67d3\u53ca\u751f\u6001\u4fee\u590d\u6548\u679c\u7684\u91cd\u8981\u6c34\u89e3\u9176\u6307\u6807\u3002\u5728\u751f\u5316\u68c0\u6d4b\u4e2d，Abbkine\u4e9a\u79d1\u56e0\u63a8\u51fa\u7684 CheKine\u2122 \u571f\u58e4\u4eae\u6c28\u9178\u6c28\u57fa\u80bd\u9176（S-LAP）\u6d3b\u6027\u68c0\u6d4b（\u5fae\u91cf\u6cd5）\u8bd5\u5242\u76d2，\u4e3a\u9ad8\u901a\u91cf\u8bc4\u4f30\u571f\u58e4\u6c2e\u5faa\u73af\u9176\u6d3b\u63d0\u4f9b\u4e86\u5feb\u6377\u53ef\u9760\u7684\u89e3\u51b3\u65b9\u6848\u3002\u672c\u6587\u5c06\u7cfb\u7edf\u9610\u8ff0 S-LAP \u7684\u751f\u6001\u5b66\u4f5c\u7528\u3001\u6d4b\u5b9a\u65b9\u6cd5\u5bf9\u6bd4\u3001\u5b9e\u64cd\u89c4\u8303\u4e0e\u9009\u8d2d\u7b56\u7565\u3002<\/p>\n

\u4e00\u3001S-LAP\u7684\u751f\u6001\u5b66\u610f\u4e49\u4e0e\u663e\u8272\u53cd\u5e94\u673a\u5236<\/h3>\n

\u571f\u58e4\u5fae\u751f\u7269\u5728\u6c2e\u9650\u5236\u6761\u4ef6\u4e0b\u4f1a\u589e\u52a0 S-LAP \u7684\u5206\u6ccc，\u4ee5\u52a0\u901f\u5916\u6e90\u86cb\u767d\u8d28\u548c\u591a\u80bd\u7684\u964d\u89e3，\u56e0\u6b64 S-LAP \u88ab\u5e7f\u6cdb\u7528\u4f5c\u201c\u571f\u58e4\u5fae\u751f\u7269\u6c2e\u9650\u5236（N-limitation）\u201d\u7684\u654f\u611f\u6307\u793a\u7269\u3002<\/p>\n

\u5b9e\u9a8c\u5ba4\u6d4b\u5b9a S-LAP \u6d3b\u6027\u6700\u7ecf\u5178\u7684\u5316\u5b66\u6bd4\u8272\u539f\u7406\u4e3a L-\u4eae\u6c28\u9178-\u5bf9\u785d\u57fa\u82ef\u80fa（L-LpNA）\u6c34\u89e3\u6cd5：<\/p>\n

  • \u5e95\u7269\u4e13\u4e00\u6027\u6c34\u89e3：\u4ee5\u5408\u6210\u5e95\u7269 L-\u4eae\u6c28\u9178-\u5bf9\u785d\u57fa\u82ef\u80fa（L-LpNA）\u4e3a\u53cd\u5e94\u5e95\u7269\u3002\u5728\u9002\u5b9c\u7684\u6e29\u5ea6\u548c pH \u7f13\u51b2\u4f53\u7cfb\u4e2d，\u571f\u58e4\u4e2d\u7684 S-LAP \u50ac\u5316 L-LpNA \u7684\u80bd\u952e\u65ad\u88c2，\u751f\u6210 \u5bf9\u785d\u57fa\u82ef\u80fa（p-Nitroaniline，\u7b80\u79f0 pNA） \u548c L-\u4eae\u6c28\u9178\u3002<\/li>\n
  • \u663e\u8272\u4e0e\u5438\u6536\u5149\u8c31：\u6e38\u79bb\u51fa\u6765\u7684 pNA \u5728 neutral\/weakly alkaline \u6761\u4ef6\u4e0b\u5448\u9ec4\u8272，\u5e76\u5728 400\u2013405 nm \u6ce2\u957f \u5904\u5177\u6709\u5f3a\u70c8\u7684\u7279\u5f81\u5438\u6536\u5cf0\u3002<\/li>\n
  • \u6d3b\u6027\u5b9a\u4e49\u4e0e\u6362\u7b97：\u5728\u4e00\u5b9a\u8303\u56f4\u5185，405 nm \u5904\u7684\u5438\u5149\u5ea6\u589e\u52a0\u503c（\u0394A405）\u4e0e\u751f\u6210\u7684 pNA \u6469\u5c14\u91cf\u6210\u6b63\u6bd4\u3002\u901a\u8fc7\u6d4b\u5b9a\u5355\u4f4d\u65f6\u95f4\u5185\u751f\u6210\u7684 pNA \u91cf，\u5373\u53ef\u8ba1\u7b97\u51fa\u5355\u4f4d\u5e72\u91cd\u571f\u58e4\u4e2d S-LAP \u7684\u9176\u6d3b\u6027\u5355\u4f4d（U\/g \u5e72\u91cd）\u3002<\/li>\n

    \u4e8c\u3001\u5e38\u89c1\u571f\u58e4\u6c2e\u5faa\u73af\u9176\u6d3b\u6027\u68c0\u6d4b\u65b9\u6cd5\u7684\u5ba2\u89c2\u5bf9\u6bd4<\/h3>\n

    \u8bc4\u4f30\u571f\u58e4\u6c2e\u8f6c\u5316\u4e0e\u86cb\u767d\u8d28\u964d\u89e3\u80fd\u529b\u65f6，\u79d1\u7814\u4eba\u5458\u53ef\u6839\u636e\u5e95\u7269\u4e0e\u4fe1\u53f7\u68c0\u6d4b\u624b\u6bb5\u9009\u62e9\u4e0d\u540c\u7684\u8bc4\u4f30\u65b9\u6cd5：<\/p>\n

      \n
    • pNA \u5fae\u91cf\u6bd4\u8272\u6cd5（L-LpNA 405 nm \u6cd5）\u539f\u7406\u4e0e\u9002\u7528\u6027：\u5229\u7528 L-LpNA \u4f5c\u4e3a\u5e95\u7269，\u901a\u8fc7\u6bd4\u8272\u6cd5\u5b9a\u91cf\u91ca\u653e\u7684 pNA（405 nm）\u3002\u6280\u672f\u4f18\u52bf：\u5e95\u7269\u4e13\u4e00\u6027\u5f3a，\u53cd\u5e94\u663e\u8272\u7a33\u5b9a；\u91c7\u7528 96 \u5b54\u677f\u5fae\u91cf\u5316\u8bbe\u8ba1，\u64cd\u4f5c\u7b80\u4fbf，\u6781\u9002\u5408\u5927\u6279\u91cf\u571f\u58e4\u6837\u54c1\u7684\u5e73\u884c\u7b5b\u9009\u3002\u5c40\u9650\u6027\u4e0e\u8fb9\u754c：\u571f\u58e4\u6d78\u51fa\u6db2\u4e2d\u7684\u8150\u6b96\u9178\u548c\u9897\u7c92\u7269\u4f1a\u5728 405 nm \u5904\u5f15\u5165\u80cc\u666f\u5438\u6536，\u5fc5\u987b\u4e25\u683c\u8bbe\u7f6e\u201c\u571f\u58e4\u80cc\u666f\u5bf9\u7167\u201d\u4ee5\u6d88\u9664\u5e72\u6270\u3002<\/li>\n
    • AMC \u8367\u5149\u5e95\u7269\u6cd5（L-\u4eae\u6c28\u9178-7-\u6c28\u57fa-4-\u7532\u57fa\u9999\u8c46\u7d20\u6cd5）\u539f\u7406\u4e0e\u9002\u7528\u6027：\u5229\u7528 Leu-AMC \u4e3a\u8367\u5149\u5e95\u7269，\u68c0\u6d4b\u9176\u89e3\u91ca\u653e\u7684 AMC \u8367\u5149（Ex 365 nm \/ Em 450 nm）\u3002\u6280\u672f\u4f18\u52bf：\u7075\u654f\u5ea6\u6781\u9ad8，\u5e95\u7269\u6d88\u8017\u5c11，\u975e\u5e38\u9002\u5408\u5fae\u91cf\u571f\u58e4（\u5982\u6839\u9645\u5fae\u533a\u571f\u3001\u5fae\u56e2\u805a\u4f53）\u7684\u9176\u6d3b\u6d4b\u5b9a\u3002\u5c40\u9650\u6027\u4e0e\u8fb9\u754c：\u571f\u58e4\u8150\u6b96\u9178\u5bf9\u8367\u5149\u5177\u6709\u8f83\u5f3a\u7684\u731d\u706d\u6548\u5e94（Quenching effect），\u8ba1\u7b97\u65f6\u9700\u9488\u5bf9\u6bcf\u4e2a\u571f\u6837\u7ed8\u5236\u8367\u5149\u731d\u706d\u5bf9\u7167\u66f2\u7ebf，\u8ba1\u7b97\u8f83\u4e3a\u7e41\u7410\u3002<\/li>\n
    • \u831a\u4e09\u916e\u6bd4\u8272\u6cd5（\u5929\u7136\u86cb\u767d\u6c34\u89e3\u6cd5）\u539f\u7406\u4e0e\u9002\u7528\u6027：\u4ee5\u916a\u86cb\u767d\u6216\u660e\u80f6\u4e3a\u5929\u7136\u5e95\u7269，\u5b75\u80b2\u540e\u7528\u831a\u4e09\u916e\u663e\u8272\u6d4b\u5b9a\u91ca\u653e\u7684\u603b\u6e38\u79bb\u6c28\u57fa\u9178\u3002\u6280\u672f\u4f18\u52bf：\u4f7f\u7528\u7684\u662f\u5929\u7136\u86cb\u767d\u8d28\u5e95\u7269，\u66f4\u63a5\u8fd1\u81ea\u7136\u754c\u4e2d\u7684\u771f\u5b9e\u751f\u6001\u8fc7\u7a0b\u3002\u5c40\u9650\u6027\u4e0e\u8fb9\u754c：\u5b75\u80b2\u65f6\u95f4\u957f（\u901a\u5e38\u9700\u8981\u6570\u5c0f\u65f6\u81f3\u5341\u51e0\u5c0f\u65f6），\u4e14\u571f\u58e4\u672c\u5e95\u6e38\u79bb\u6c28\u57fa\u9178\u542b\u91cf\u9ad8，\u80cc\u666f\u5e72\u6270\u5927，\u7075\u654f\u5ea6\u4f4e\u3002<\/li>\n<\/ul>\n

      \u4e09\u3001\u5b9e\u64cd\u6307\u5357：\u571f\u58e4\u6837\u672c\u524d\u5904\u7406\u4e0e\u5fae\u91cf\u6bd4\u8272\u6b65\u9aa4<\/h3>\n

      \u3010\u5b89\u5168\u64cd\u4f5c\u8b66\u793a\u3011<\/h4>\n

      \u672c\u5b9e\u9a8c\u6240\u7528\u7684\u5e95\u7269\u6eb6\u6db2\u53ca pNA \u4ea7\u7269\u542b\u6709\u6709\u673a\u6eb6\u5242\u53ca\u5fae\u91cf\u6709\u673a\u80fa\u6210\u5206，\u5177\u6709\u8f7b\u5fae\u7684\u751f\u7269\u6bd2\u6027\u4e0e\u523a\u6fc0\u6027\u3002\u5b9e\u9a8c\u64cd\u4f5c\u5fc5\u987b\u5728\u901a\u98ce\u826f\u597d\u7684\u5b9e\u9a8c\u5ba4\u5185\u8fdb\u884c，\u64cd\u4f5c\u4eba\u5458\u987b\u7a7f\u6234\u5b9e\u9a8c\u670d\u3001\u4e00\u6b21\u6027\u4e01\u8148\u624b\u5957\u4e0e\u62a4\u76ee\u955c\u3002\u907f\u514d\u8bd5\u5242\u76f4\u63a5\u63a5\u89e6\u76ae\u80a4\u6216\u5438\u5165\u84b8\u6c7d\u3002<\/p>\n

      1. \u571f\u58e4\u6837\u672c\u7684\u524d\u5904\u7406<\/h4>\n
        \n
      • \u91c7\u7b5b\u4e0e\u4fdd\u5b58：\u91c7\u96c6\u8868\u5c42\u571f\u58e4（0\u201310 cm \u6216 0\u201320 cm），\u5254\u9664\u690d\u7269\u7ec6\u6839\u3001\u77f3\u5757\u53ca\u6606\u866b\u6b8b\u4f53\u3002\u65b0\u9c9c\u571f\u58e4\u901a\u8fc7 20\u201340 \u76ee\u7b5b\u3002\u5efa\u8bae\u4f18\u5148\u4f7f\u7528\u65b0\u9c9c\u6e7f\u6da6\u571f\u58e4\u8fdb\u884c\u6d4b\u5b9a（\u4e8e 4 \u2103 \u4fdd\u5b58\u5e76\u5728 1 \u5468\u5185\u5b8c\u6210\u68c0\u6d4b），\u4ee5\u771f\u5b9e\u53cd\u6620\u5fae\u751f\u7269\u80de\u5916\u9176\u6d3b\u6027\u3002\u82e5\u4f7f\u7528\u98ce\u5e72\u571f，\u9700\u6ce8\u610f\u9176\u6d3b\u6027\u4f1a\u6709\u4e00\u5b9a\u7a0b\u5ea6\u7684\u8870\u51cf\u3002<\/li>\n
      • \u542b\u6c34\u91cf\u6d4b\u5b9a：\u53e6\u53d6\u4e00\u4efd\u540c\u6279\u6b21\u571f\u58e4，\u79f0\u91cd\u540e\u4e8e 105 \u2103 \u70d8\u5e72\u81f3\u6052\u91cd，\u8ba1\u7b97\u571f\u58e4\u542b\u6c34\u91cf（%），\u7528\u4e8e\u6700\u7ec8\u9176\u6d3b\u6027\u7684\u201c\u5e72\u91cd（Dry Weight）\u201d\u6362\u7b97\u3002<\/li>\n<\/ul>\n

        2. 96\u5b54\u677f\u5fae\u91cf\u6cd5\u6bd4\u8272\u6b65\u9aa4<\/h4>\n
      • \u6807\u51c6\u54c1\u914d\u5236：\u7528\u7f13\u51b2\u6db2\u5c06 pNA \u6807\u51c6\u54c1\u68af\u7ea7\u7a00\u91ca\u4e3a 0\u300110\u300120\u300140\u300180\u3001160 \u03bcmol\/L \u7684\u68af\u5ea6\u6807\u51c6\u6eb6\u6db2\u3002<\/li>\n
      • \u8bbe\u7f6e\u53cd\u5e94\u5206\u7ec4（\u79f0\u53d6\u571f\u58e4\u4e8e\u79bb\u5fc3\u7ba1\u4e2d）：\u6d4b\u5b9a\u7ba1：\u79f0\u53d6\u98ce\u5e72\u6216\u9c9c\u571f 0.05 g，\u52a0\u5165\u9002\u91cf\u7f13\u51b2\u6db2\u53ca \u5e95\u7269 L-LpNA \u6eb6\u6db2\u3002\u571f\u58e4\u80cc\u666f\u7ba1：\u79f0\u53d6\u98ce\u5e72\u6216\u9c9c\u571f 0.05 g，\u52a0\u5165\u9002\u91cf\u7f13\u51b2\u6db2\u53ca \u84b8\u998f\u6c34\/\u65e0\u5e95\u7269\u7f13\u51b2\u6db2（\u7528\u4e8e\u6263\u9664\u571f\u58e4\u6d78\u51fa\u6db2\u672c\u8eab\u7684\u8272\u5ea6\u4e0e\u6d51\u6d4a）\u3002\u65e0\u571f\u7a7a\u767d\u7ba1：\u4e0d\u52a0\u571f\u6837，\u4ec5\u52a0\u5165\u7f13\u51b2\u6db2\u4e0e \u5e95\u7269 L-LpNA \u6eb6\u6db2（\u7528\u4e8e\u6263\u9664\u5e95\u7269\u7684\u975e\u9176\u81ea\u6c34\u89e3\u80cc\u666f）\u3002<\/li>\n
      • \u6052\u6e29\u5b75\u80b2：\u5145\u5206\u6df7\u5300\u540e，\u7f6e\u4e8e 37 \u2103 \u6052\u6e29\u632f\u8361\u6447\u5e8a\u6216\u6c34\u6d74\u4e2d\u907f\u5149\u53cd\u5e94 1\u20132 \u5c0f\u65f6\u3002<\/li>\n
      • \u79bb\u5fc3\u4e0e\u6bd4\u8272：\u53cd\u5e94\u7ed3\u675f\u540e，\u4e8e 10,000 rpm \u79bb\u5fc3 5 \u5206\u949f\u3002\u5438\u53d6 200 \u03bcL \u6e05\u4eae\u4e0a\u6e05\u6db2 \u8f6c\u79fb\u81f3 96 \u5b54\u900f\u660e\u5fae\u5b54\u677f\u4e2d，\u5229\u7528\u9176\u6807\u4eea\u8bfb\u53d6 405 nm \u6ce2\u957f \u4e0b\u7684\u5438\u5149\u5ea6 A\u3002<\/li>\n
      • \u6d3b\u6027\u8ba1\u7b97：$\\\\Delta A = (A_{\\\\text{\u6d4b\u5b9a}} – A_{\\\\text{\u571f\u58e4\u80cc\u666f}}) – A_{\\\\text{\u65e0\u571f\u7a7a\u767d}}$\u7ed3\u5408\u6807\u51c6\u66f2\u7ebf\u4e0e\u571f\u58e4\u5e72\u91cd\u6362\u7b97\u5f97\u51fa S-LAP \u6d3b\u6027（U\/g \u5e72\u91cd）\u3002<\/li>\n

        \u56db\u3001\u5e38\u89c1\u5b9e\u9a8c\u96be\u9898\u4e0e\u907f\u5751\u6307\u5357<\/h3>\n
          \n
        • \u95ee\u9898\u4e00：\u571f\u58e4\u80cc\u666f\u7ba1\u5438\u5149\u5ea6\u6781\u9ad8，\u5bfc\u81f4 \u0394A \u6781\u5c0f\u6216\u8ba1\u7b97\u51fa\u73b0\u8d1f\u503c\u539f\u56e0\u5206\u6790：\u571f\u58e4\u5bcc\u542b\u9ad8\u6d53\u5ea6\u53ef\u6eb6\u6027\u8150\u6b96\u9178（\u5982\u9ed1\u571f\u3001\u6cbc\u6cfd\u571f）\u6216\u6ce5\u6c99\u9897\u7c92\u60ac\u6d6e，\u5728 405 nm \u5904\u4ea7\u751f\u5f3a\u70c8\u80cc\u666f\u5438\u6536；\u6216\u8005\u5e95\u7269 L-LpNA \u5728\u914d\u5236\u65f6\u53d1\u751f\u81ea\u6c34\u89e3\u3002\u89e3\u51b3\u65b9\u6848：\u5fc5\u987b\u4e25\u683c\u4fdd\u7559\u6bcf\u4e00\u4e2a\u571f\u58e4\u6837\u672c\u5bf9\u5e94\u7684\u201c\u571f\u58e4\u80cc\u666f\u7ba1\u201d；\u82e5\u79bb\u5fc3\u540e\u4e0a\u6e05\u6db2\u4ecd\u6df7\u6d4a，\u53ef\u5ef6\u957f\u79bb\u5fc3\u65f6\u95f4（\u5982 13,000 rpm \u79bb\u5fc3 8 min）\u6216\u4f7f\u7528 0.22 \u03bcm \u9488\u5934\u8fc7\u6ee4\u5668\u6ee4\u8fc7\u540e\u518d\u6bd4\u8272\u3002<\/li>\n
        • \u95ee\u9898\u4e8c：\u6d4b\u5b9a\u5438\u5149\u5ea6\u8d85\u51fa\u4e86\u5149\u5bc6\u5ea6\u6700\u5927\u7ebf\u6027\u8303\u56f4（A > 2.0）\u539f\u56e0\u5206\u6790：\u571f\u58e4\u5fae\u751f\u7269\u91cf\u4e30\u5bcc（\u5982\u80a5\u6c83\u519c\u7530\u6216\u68ee\u6797\u8868\u5c42\u571f），S-LAP \u9176\u6d3b\u6027\u6781\u5f3a，\u5bfc\u81f4\u5e95\u7269\u5728\u5b75\u80b2\u65f6\u95f4\u5185\u88ab\u8fc7\u5ea6\u6d88\u8017\u3002\u89e3\u51b3\u65b9\u6848：\u9002\u5f53\u7f29\u77ed\u5b75\u80b2\u65f6\u95f4（\u5982\u7531 60 \u5206\u949f\u7f29\u77ed\u81f3 20\u201330 \u5206\u949f），\u6216\u8005\u51cf\u5c11\u79f0\u6837\u91cf（\u5982\u51cf\u5c11\u81f3 0.02 g）\u91cd\u65b0\u6d4b\u5b9a\u3002<\/li>\n
        • \u95ee\u9898\u4e09：\u5e73\u884c\u7ba1\u4e4b\u95f4\u5dee\u5f02\u5927（CV > 15%）\u539f\u56e0\u5206\u6790：\u571f\u6837\u672a\u5145\u5206\u7814\u78e8\u8fc7\u7b5b\u5bfc\u81f4\u79f0\u6837\u4e0d\u5747\u5300；\u6216\u8005\u5b75\u80b2\u8fc7\u7a0b\u4e2d\u571f\u6837\u6c89\u964d，\u672a\u80fd\u4e0e\u5e95\u7269\u5145\u5206\u63a5\u89e6\u3002\u89e3\u51b3\u65b9\u6848：\u571f\u6837\u5fc5\u987b\u8fc7 20\u201340 \u76ee\u7b5b\u5e76\u5145\u5206\u6df7\u5300；\u5b75\u80b2\u8fc7\u7a0b\u5efa\u8bae\u4f7f\u7528\u6052\u6e29\u632f\u8361\u6447\u5e8a，\u4fdd\u6301\u571f\u7c92\u5904\u4e8e\u60ac\u6d6e\u72b6\u6001\u3002<\/li>\n<\/ul>\n

          \u4e94\u3001\u6838\u5fc3\u6280\u672f\u53c2\u6570\u4e0e\u9009\u8d2d\u6307\u5357<\/h3>\n

          \u5728\u91c7\u8d2d\u571f\u58e4\u4eae\u6c28\u9178\u6c28\u57fa\u80bd\u9176（S-LAP）\u6d3b\u6027\u68c0\u6d4b\u8bd5\u5242\u76d2\u65f6，\u5efa\u8bae\u79d1\u7814\u4eba\u5458\u5173\u6ce8\u4ee5\u4e0b\u6307\u6807：<\/p>\n

        • \u5e95\u7269\u6eb6\u89e3\u5ea6\u4e0e\u7eaf\u5ea6：L-LpNA \u5728\u6c34\u76f8\u4e2d\u7684\u6eb6\u89e3\u5ea6\u8f83\u4f4e\u3002\u9ad8\u54c1\u8d28\u8bd5\u5242\u76d2\u4f1a\u63d0\u4f9b\u4e13\u7528\u7684\u52a9\u6eb6\u5242\u4e0e\u5e95\u7269\u4fdd\u62a4\u4f53\u7cfb，\u786e\u4fdd\u5e95\u7269\u5b8c\u5168\u6eb6\u89e3\u4e14\u80cc\u666f\u5438\u5149\u5ea6\u4f4e\u3002<\/li>\n
        • \u6263\u9664\u80cc\u666f\u8bbe\u8ba1\u7684\u5408\u7406\u6027：\u786e\u8ba4\u8bd5\u5242\u76d2\u8bf4\u660e\u4e66\u662f\u5426\u63d0\u4f9b\u4e86\u5b8c\u5584\u7684\u201c\u571f\u58e4\u80cc\u666f\u6263\u9664\u201d\u4e0e\u201c\u5e95\u7269\u81ea\u89e3\u6790\u6263\u9664\u201d\u53cc\u91cd\u5bf9\u7167\u8bbe\u8ba1，\u8fd9\u662f\u786e\u4fdd\u9ad8\u6709\u673a\u8d28\u571f\u58e4\u6570\u636e\u771f\u5b9e\u6027\u7684\u5173\u952e\u3002<\/li>\n
        • \u5fae\u91cf\u5316\u4e0e\u9ad8\u901a\u91cf\u9002\u914d：\u9009\u62e9\u4e13\u4e3a 96 \u5b54\u677f\u8bbe\u8ba1\u7684\u5fae\u91cf\u8bd5\u5242\u76d2，\u5c06\u79bb\u5fc3\u7ba1\u79bb\u5fc3\u4e0e\u5fae\u5b54\u677f\u6bd4\u8272\u76f8\u7ed3\u5408，\u5927\u5e45\u8282\u7701\u8bd5\u5242\u7528\u91cf\u5e76\u63d0\u9ad8\u6279\u91cf\u68c0\u6d4b\u6548\u7387\u3002<\/li>\n
        • \u573a\u666f\u9009\u578b\u63a8\u8350：\u5bf9\u4e8e\u571f\u58e4\u6c2e\u7d20\u77ff\u5316\u3001\u6709\u673a\u8d28\u5206\u89e3\u53ca\u5fae\u751f\u7269\u4ee3\u8c22\u9650\u5236\u7814\u7a76，S-LAP \u662f\u6838\u5fc3\u6307\u6807；\u5efa\u8bae\u4e0e\u571f\u58e4\u8132\u9176（S-UE）\u3001\u571f\u58e4\u785d\u9178\u8fd8\u539f\u9176（S-NR）\u4ee5\u53ca\u571f\u58e4 N-\u4e59\u9170-\u03b2-D-\u8461\u8404\u7cd6\u82f7\u9176（S-NAG）\u8bd5\u5242\u76d2\u8054\u5408\u9009\u8d2d，\u6784\u5efa\u5b8c\u6574\u7684\u571f\u58e4\u6c2e\u5faa\u73af\u9176\u529f\u80fd\u8c31\u3002<\/li>\n

          \u516d\u3001\u603b\u7ed3<\/h3>\n

          \u571f\u58e4\u4eae\u6c28\u9178\u6c28\u57fa\u80bd\u9176（S-LAP）\u4f5c\u4e3a\u571f\u58e4\u6709\u673a\u6c2e\u77ff\u5316\u7684\u5173\u952e\u9650\u901f\u9176，\u5176\u6d3b\u6027\u7684\u51c6\u786e\u6d4b\u5b9a\u5bf9\u4e8e\u8bc4\u4ef7\u571f\u58e4\u6c2e\u7d20\u4f9b\u5e94\u80fd\u529b\u4e0e\u751f\u6001\u7cfb\u7edf\u529f\u80fd\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002\u5728\u5b9e\u9a8c\u4e2d\u4e25\u683c\u63a7\u5236\u571f\u6837\u5747\u5300\u5ea6\u3001\u7cbe\u786e\u8bbe\u7f6e\u571f\u58e4\u4e0e\u5e95\u7269\u53cc\u91cd\u80cc\u666f\u6263\u9664\u4ee5\u53ca\u638c\u63e1\u9002\u5b9c\u7684\u5b75\u80b2\u65f6\u95f4，\u662f\u83b7\u53d6\u9ad8\u91cd\u73b0\u6027\u6570\u636e\u7684\u6838\u5fc3\u3002\u4f5c\u4e3a\u751f\u547d\u79d1\u5b66\u4e0e\u751f\u6001\u751f\u5316\u5de5\u5177\u9886\u57df\u7684\u4e13\u4e1a\u54c1\u724c，Abbkine\u4e9a\u79d1\u56e0\u81f4\u529b\u4e8e\u6301\u7eed\u4f18\u5316\u571f\u58e4\u9176\u6d3b\u68c0\u6d4b\u6280\u672f，\u4e3a\u5168\u7403\u751f\u6001\u5b66\u4e0e\u519c\u5b66\u7814\u7a76\u4eba\u5458\u63d0\u4f9b\u4f18\u8d28\u7684\u8bd5\u5242\u76d2\u4e0e\u6280\u672f\u4fdd\u969c\u3002<\/p><\/p>\n","protected":false},"excerpt":{"rendered":"

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