{"id":89542,"date":"2026-08-03T08:24:55","date_gmt":"2026-08-03T00:24:55","guid":{"rendered":"https:\/\/www.wsisp.com\/helps\/89542.html"},"modified":"2026-08-03T08:24:55","modified_gmt":"2026-08-03T00:24:55","slug":"%e5%a6%82%e4%bd%95%e8%be%a8%e5%88%ab%e5%8e%9f%e4%bb%a3%e7%bb%86%e8%83%9e%ef%bc%9f4-%e5%a5%97%e9%89%b4%e5%ae%9a%e6%96%b9%e6%b3%95%ef%bc%8c%e8%bd%bb%e6%9d%be%e5%88%86%e8%be%a8%e5%8e%9f%e4%bb%a3%e7%bb%86","status":"publish","type":"post","link":"https:\/\/www.wsisp.com\/helps\/89542.html","title":{"rendered":"\u5982\u4f55\u8fa8\u522b\u539f\u4ee3\u7ec6\u80de\uff1f4 \u5957\u9274\u5b9a\u65b9\u6cd5\uff0c\u8f7b\u677e\u5206\u8fa8\u539f\u4ee3\u7ec6\u80de & \u7ec6\u80de\u7cfb"},"content":{"rendered":"

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1. \u539f\u4ee3\u7ec6\u80de\u7684\u5f62\u6001\u7279\u5f81<\/span><\/h3>\n\n\n\n\n\n\n\n\n\n\n
\n

\u7ec6\u80de\u7c7b\u578b<\/span><\/p>\n<\/td>\n

\n

\u5178\u578b\u5f62\u6001<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u795e\u7ecf\u5143<\/span><\/p>\n<\/td>\n

\n

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\n

\u5c0f\u80f6\u8d28\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

\u5448\u73b0\u963f\u7c73\u5df4\u6837（\u5706\u5f62\/\u5375\u5706\u5f62，\u6709\u4f2a\u8db3）\u6216\u5206\u679d\u72b6（\u591a\u7ea7\u7ec6\u957f\u5206\u652f）\u5f62\u6001<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u661f\u5f62\u80f6\u8d28\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

\u5448\u661f\u5f62\u6216\u4e0d\u89c4\u5219\u591a\u8fb9\u5f62，\u6709\u5927\u91cf\u7a81\u8d77<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u6210\u7ea4\u7ef4\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

\u957f\u68ad\u5f62，\u4e24\u7aef\u5c16，\u6392\u5217\u5448\u65cb\u6da1\u72b6\u6216\u5e73\u884c\u675f\u72b6<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u4e0a\u76ae\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

\u5448\u94fa\u8def\u77f3\u6837（\u9e45\u5375\u77f3\u72b6），\u6392\u5217\u7d27\u5bc6\u89c4\u6574，\u7ec6\u80de\u8fb9\u754c\u6e05\u6670<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u5185\u76ae\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

\u5448\u77ed\u68ad\u5f62\u6216\u591a\u8fb9\u5f62，\u5448\u9e45\u5375\u77f3\u6837\u5355\u5c42\u94fa\u5c55<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u8f6f\u9aa8\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

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\"\"<\/p>\n

\u56fe1 \u5e38\u89c1\u539f\u4ee3\u7ec6\u80de\u5f62\u6001\u7279\u5f81（\u6b66\u6c49\u4e91\u514b\u9686\u539f\u4ee3\u7ec6\u80de）<\/span><\/p>\n

2. \u7ec6\u80de\u7cfb\u7684\u5f62\u6001\u7279\u5f81<\/span><\/h3>\n

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\u5178\u578b\u6848\u4f8b：<\/span><\/p>\n

HT22（\u6c38\u751f\u5316\u5c0f\u9f20\u6d77\u9a6c\u795e\u7ecf\u5143）：\u5b8c\u5168\u6ca1\u6709\u8f74\u7a81\/\u6811\u7a81，\u5c31\u662f\u5747\u4e00\u7684\u68ad\u5f62\u6216\u7c7b\u5706\u5f62<\/span><\/p>\n

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HL-1\/H9C2 (\u5fc3\u808c\u7ec6\u80de）:\u7ec6\u80de\u5f62\u6001\u51e0\u4e4e\u90fd\u662f\u68ad\u5f62，\u65e0\u81ea\u4e3b\u640f\u52a8\u80fd\u529b<\/span><\/p>\n

HeLa：\u591a\u5f62\u6027，\u5806\u79ef\u751f\u957f，\u6838\u5927\u6df1\u67d3<\/span><\/p>\n

\"\"<\/p>\n

\u56fe2 \u7ec6\u80de\u7cfbSH-SY5Y\u7684\u5f62\u6001\u7279\u5f81（\u6765\u81ea\u4e8eATCC）<\/span><\/p>\n

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\"\"<\/p>\n

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1. \u4f20\u4ee3\u6b21\u6570\u4e0e\u589e\u6b96\u6f5c\u529b<\/span><\/h4>\n\n\n\n\n\n\n
\n

\u6307\u6807<\/span><\/p>\n<\/td>\n

\n

\u539f\u4ee3\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

\u7ec6\u80de\u7cfb<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

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\n

\u901a\u5e38\u226410\u4ee3<\/span><\/span><\/p>\n<\/td>\n

\n

>50\u4ee3，\u53ef\u65e0\u9650<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u57f9\u517b\u5bff\u547d<\/span><\/span><\/p>\n<\/td>\n

\n

1\u5468~1\u4e2a\u6708<\/span><\/p>\n<\/td>\n

\n

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\n

\u8870\u8001\u8868\u73b0<\/span><\/span><\/p>\n<\/td>\n

\n

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\n

\u6c38\u8fdc\u5e74\u8f7b，\u65e0\u8870\u8001\u8ff9\u8c61<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/p>\n

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2. \u500d\u589e\u65f6\u95f4\u4e0e\u751f\u957f\u66f2\u7ebf<\/span><\/h4>\n

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3. \u63a5\u89e6\u6291\u5236（\u6700\u7ecf\u5178\u7684\u533a\u522b！）<\/span><\/h4>\n

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\u5b9e\u9a8c\u65b9\u6cd5<\/span>：\u5c06\u7ec6\u80de\u63a5\u79cd\u81f36\u5b54\u677f，\u6bcf\u65e5\u8ba1\u6570，\u7ed8\u5236\u751f\u957f\u66f2\u7ebf，\u5e76\u5728\u6c47\u5408\u540e\u7ee7\u7eed\u89c2\u5bdf3~5\u5929，\u5224\u65ad\u662f\u5426\u51fa\u73b0\u5806\u79ef\u751f\u957f\u3002<\/span><\/span><\/p>\n

\"\"<\/p>\n

\u56fe5 \u539f\u4ee3\u7ec6\u80de\u4e0e\u7ec6\u80de\u7cfb\u751f\u957f\u7279\u6027<\/span><\/p>\n

4. \u951a\u5b9a\u4f9d\u8d56\u6027（\u9488\u5bf9\u4e8e\u8d34\u58c1\u7ec6\u80de）<\/span><\/h4>\n

\u539f\u4ee3\u7ec6\u80de：\u90e8\u5206\u539f\u4ee3\u7ec6\u80de\u4e25\u683c\u7684\u951a\u5b9a\u4f9d\u8d56\u6027，\u987b\u8d34\u9644\u4e8e\u7ecf\u7ec6\u80de\u5916\u57fa\u8d28（\u5982\u80f6\u539f\u3001\u660e\u80f6\u3001\u591a\u805a\u8d56\u6c28\u9178\u7b49）\u5305\u88ab\u7684\u57f9\u517b\u8868\u9762\u624d\u80fd\u5b58\u6d3b\u548c\u589e\u6b96；<\/span><\/p>\n

\u7ec6\u80de\u7cfb：\u951a\u5b9a\u4f9d\u8d56\u6027\u51cf\u5f31，\u90e8\u5206\u7ec6\u80de\u7cfb\u53ef\u9002\u5e94\u60ac\u6d6e\u57f9\u517b（\u5982\u60ac\u6d6e\u9002\u5e94\u7684CHO\u3001293F\u7ec6\u80de）\u3002<\/span><\/p>\n

5. \u63a5\u79cd\u5b58\u6d3b\u7387\u4e0e\u514b\u9686\u5f62\u6210\u80fd\u529b<\/span><\/h4>\n

\u539f\u4ee3\u7ec6\u80de<\/span>：\u4f4e\u5bc6\u5ea6\u63a5\u79cd\u65f6\u5b58\u6d3b\u7387\u4f4e，\u5355\u7ec6\u80de\u514b\u9686\u5f62\u6210\u6548\u7387\u6781\u4f4e（<1%）<\/span>，<\/span>\u63a5\u79cd\u65f6\u9700\u8981\u628a\u63e1\u4e00\u5b9a\u7684\u7ec6\u80de\u5bc6\u5ea6<\/span><\/span><\/p>\n

\u7ec6\u80de\u7cfb<\/span>：\u4f4e\u5bc6\u5ea6\u63a5\u79cd\u5b58\u6d3b\u7387\u9ad8，\u514b\u9686\u5f62\u6210\u80fd\u529b\u5f3a（>10%，\u90e8\u5206\u53ef\u8fbe50%\u4ee5\u4e0a）<\/span><\/span><\/p>\n

\u65b9\u6cd5\u4e09：\u9057\u4f20\u4e0e\u8868\u578b\u2014\u7279\u5f02\u6027\u6807\u5fd7\u7269\u68c0\u6d4b（\u6700\u6709\u529b\u7684\u8eab\u4efd\u8bc1\u636e）<\/span><\/h2>\n

\u201c\u957f\u5f97\u50cf\u201d\u8fd8\u4e0d\u591f，\u8981\u770b\u5b83\u6709\u6ca1\u6709\u201c\u8eab\u4efd\u8bc1\u201d\u2014\u2014\u7ec4\u7ec7\u7279\u5f02\u6027\u7684\u5206\u5316\u6807\u5fd7\u7269\u3002<\/span><\/p>\n

1\u3001\u5e38\u7528\u68c0\u6d4b\u6280\u672f：<\/span><\/h4>\n

\u2460\u514d\u75ab\u8367\u5149（IF）：\u7279\u5f02\u6027\u6807\u5fd7\u7269\u6297\u4f53<\/span><\/p>\n

\u2461\u6d41\u5f0f（FACS）：\u7279\u5f02\u6027\u6807\u5fd7\u7269\u6d41\u5f0f\u6297\u4f53<\/span><\/p>\n

\u2462\u7279\u5f02\u6027\u67d3\u8272：\u5982\u963f\u5229\u65b0\u84dd\u67d3\u8272\u3001\u7532\u82ef\u80fa\u84dd\u67d3\u8272\u3001PAS\u7cd6\u539f\u67d3\u8272\u3001\u831c\u7d20\u7ea2\u67d3\u8272\u3001\u6cb9\u7ea2O\u3001ALP\u67d3\u8272<\/span><\/p>\n

\u2463\u529f\u80fd\u9274\u5b9a：\u6210\u9aa8\u5206\u5316\u3001\u6210\u8102\u5206\u5316\u3001\u6210\u8f6f\u9aa8\u5206\u5316<\/span><\/p>\n

\u2464\u5176\u4ed6：\u5982Western Blot\u3001qRT-PCR<\/span><\/p>\n

2. \u5404\u7c7b\u7ec6\u80de\u7684\u7279\u5f02\u6027\u6807\u5fd7\u7269<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n
\n

\u7ec6\u80de\u7c7b\u578b<\/span><\/p>\n<\/td>\n

\n

\u539f\u4ee3\u7ec6\u80de（<\/span>\u9633\u6027\u7684\u6807\u5fd7\u7269<\/span>）<\/span><\/p>\n<\/td>\n

\n

\u7ec6\u80de\u7cfb（\u5e38<\/span>\u4e22\u5931\u7684\u6807\u5fd7\u7269<\/span>）<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u795e\u7ecf\u5143<\/span><\/p>\n<\/td>\n

\n

NeuN, MAP2, \u03b2-III Tubulin<\/span><\/p>\n<\/td>\n

\n

\u4e0a\u8ff0\u6807\u5fd7\u7269\u8868\u8fbe\u663e\u8457\u964d\u4f4e\u6216\u6d88\u5931<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u5c0f\u80f6\u8d28\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

Iba1, CD11b, P2RY12, CD68<\/span><\/span><\/p>\n<\/td>\n

\n

Iba1\/P2RY12\u4e0b\u8c03，\u5f62\u6001\u76f8\u5173\u6807\u5fd7\u7269\u4e27\u5931<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u661f\u5f62\u80f6\u8d28\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

GFAP, S100\u03b2<\/span><\/p>\n<\/td>\n

\n

GFAP\u8868\u8fbe\u6539\u53d8<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u6210\u7ea4\u7ef4\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

Vimentin, Fibronectin，FSP1<\/span><\/p>\n<\/td>\n

\n

\u90e8\u5206\u95f4\u8d28\u6807\u5fd7\u7269\u6539\u53d8<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u4e0a\u76ae\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

E-cadherin, Cytokeratin, ZO-1<\/span><\/p>\n<\/td>\n

\n

\u4e0a\u76ae\u6807\u5fd7\u7269\u4e22\u5931（\u53ef\u80fd\u53d1\u751fEMT）<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u5185\u76ae\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

CD31, vWF, VE-cadherin<\/span><\/p>\n<\/td>\n

\n

\u8840\u7ba1\u7279\u5f02\u6027\u6807\u5fd7\u7269\u4e0b\u8c03<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u809d\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

Albumin, CK18, CYP450\u9176<\/span><\/p>\n<\/td>\n

\n

\u4ee3\u8c22\u9176\u6d3b\u6027\u663e\u8457\u4e0b\u964d<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u8f6f\u9aa8\u7ec6\u80de<\/span><\/span><\/p>\n<\/td>\n

\n

Collagen II, Aggrecan, SOX9<\/span><\/span><\/p>\n<\/td>\n

\n

\u5206\u5316\u6807\u5fd7\u7269\u4e22\u5931，\u53bb\u5206\u5316<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

3. \u6c38\u751f\u5316\/\u8f6c\u5316\u76f8\u5173\u6807\u5fd7\u7269（\u53cd\u5411\u8bc1\u636e）<\/h4>\n

\u9664\u4e86\u5206\u5316\u6807\u5fd7\u7269\u7684\u4e22\u5931，\u7ec6\u80de\u7cfb\u8fd8\u53ef\u80fd\u83b7\u5f97\u6216\u4e0a\u8c03\u67d0\u4e9b\u4e0e\u6c38\u751f\u5316\/\u8f6c\u5316\u76f8\u5173\u7684\u6807\u5fd7\u7269：<\/p>\n\n\n\n\n\n\n\n
\n

\u6807\u5fd7\u7269\u7c7b\u578b<\/span><\/p>\n<\/td>\n

\n

\u5177\u4f53\u6807\u5fd7\u7269<\/span><\/p>\n<\/td>\n

\n

\u539f\u4ee3\u7ec6\u80de<\/span><\/p>\n<\/td>\n

\n

\u7ec6\u80de\u7cfb<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u7aef\u7c92\u9176<\/span><\/p>\n<\/td>\n

\n

hTERT\u86cb\u767d\/\u57fa\u56e0\u8868\u8fbe<\/span><\/p>\n<\/td>\n

\n

\u6781\u4f4e\/\u9634\u6027<\/span><\/p>\n<\/td>\n

\n

\u9ad8\u8868\u8fbe<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u80bf\u7624\u6291\u5236\u86cb\u767d<\/span><\/p>\n<\/td>\n

\n

p53, pRB, p16, p21<\/span><\/p>\n<\/td>\n

\n

\u6b63\u5e38\u8868\u8fbe，\u529f\u80fd\u5b8c\u6574<\/span><\/p>\n<\/td>\n

\n

\u5e38\u53d1\u751f\u7a81\u53d8\/\u7f3a\u5931，\u529f\u80fd\u5931\u6d3b<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u589e\u6b96\u6807\u5fd7\u7269<\/span><\/p>\n<\/td>\n

\n

Ki-67, PCNA<\/span><\/p>\n<\/td>\n

\n

\u4f4e\u8868\u8fbe（\u4ec5\u5728\u589e\u6b96\u7ec6\u80de\u4e2d）<\/span><\/p>\n<\/td>\n

\n

\u9ad8\u8868\u8fbe（\u9633\u6027\u7387\u9ad8）<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u5e72\u6027\u6807\u5fd7\u7269<\/span><\/p>\n<\/td>\n

\n

SOX2, OCT4, NANOG（\u975e\u5e72\u7ec6\u80de\u6765\u6e90）<\/span><\/p>\n<\/td>\n

\n

\u4e0d\u8868\u8fbe<\/span><\/p>\n<\/td>\n

\n

\u90e8\u5206\u7ec6\u80de\u7cfb\u5f02\u5e38\u8868\u8fbe<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

4. \u5224\u5b9a\u6807\u51c6<\/span><\/span><\/span><\/h4>\n

\u539f\u4ee3\u7ec6\u80de\u786e\u8bc1\u6807\u51c6<\/span><\/span>：\u7279\u5f02\u6027\u5206\u5316\u6807\u5fd7\u7269\u9ad8\u9633\u6027\u7387（>70~90%，\u53d6\u51b3\u4e8e\u7ec6\u80de\u7c7b\u578b\u548c\u7eaf\u5ea6），\u540c\u65f6\u6c38\u751f\u5316\u6807\u5fd7\u7269（\u5982hTERT）\u9634\u6027<\/span><\/span>\u3002<\/span><\/span><\/p>\n

\u7ec6\u80de\u7cfb\u7279\u5f81<\/span><\/span>：\u5206\u5316\u6807\u5fd7\u7269\u9633\u6027\u7387\u663e\u8457\u964d\u4f4e（\u901a\u5e38<30%）\u6216\u5b8c\u5168\u4e22\u5931，\u540c\u65f6\u4f34\u968f\u6c38\u751f\u5316\/\u589e\u6b96\u6807\u5fd7\u7269\u9ad8\u8868\u8fbe<\/span><\/span>\u3002<\/span><\/span><\/p>\n

\u65b9\u6cd5\u56db：STR\u5206\u578b\u2014\u2014\u4eba\u6e90\u7ec6\u80de\u7684\u201cDNA\u8eab\u4efd\u8bc1\u201d（\u56fd\u9645\u91d1\u6807\u51c6）<\/h2>\n

\u5c31\u50cf\u4eba\u7684\u6307\u7eb9\u4e00\u6837，\u6bcf\u4e2a\u7ec6\u80de\u7cfb\u90fd\u6709\u81ea\u5df1\u7684STR\u56fe\u8c31，\u548c\u6570\u636e\u5e93\u4e00\u6bd4\u5bf9，\u8eab\u4efd\u7acb\u523b\u63ed\u6653\u3002<\/p>\n

\u5224\u5b9a\u6807\u51c6：（\u53cd\u5411\u8bc1\u660e）<\/h3>\n\n\n\n\n\n\n
\n

\u6bd4\u5bf9\u7ed3\u679c<\/span><\/p>\n<\/td>\n

\n

\u7ed3\u8bba<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u5339\u914d\u5ea6 \u2265 80%<\/span><\/p>\n<\/td>\n

\n

\u4e00\u5b9a\u6307\u7ec6\u80de\u7cfb<\/span><\/span>\u3002<\/span><\/span>\u5f85\u6d4b\u6837\u672c\u53ef\u786e\u8ba4\u4e3a\u8be5\u5df2\u77e5\u7ec6\u80de\u7cfb，\u8eab\u4efd\u9a8c\u8bc1\u901a\u8fc7<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u5339\u914d\u5ea6 < 80% \u6216\u51fa\u73b0\u6df7\u5408\u5cf0<\/span><\/p>\n<\/td>\n

\n

\u53ef\u80fd\u5b58\u5728\u9519\u8bef\u6807\u8bb0\u3001\u4ea4\u53c9\u6c61\u67d3\u6216\u6df7\u5408\u7ec6\u80de\u7fa4<\/span><\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u65e0\u5339\u914d<\/span><\/p>\n<\/td>\n

\n

\u53ef\u80fd\u4e3a\u539f\u4ee3\u7ec6\u80de\u3001\u65b0\u578b\u7ec6\u80de\u7cfb\u6216\u975e\u4eba\u6e90\u7ec6\u80de<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5173\u952e\u5e94\u7528\u4ef7\u503c：<\/h3>\n

\u5982\u679c\u4e00\u4efd\u58f0\u79f0\u662f\u201c\u539f\u4ee3\u7ec6\u80de\u201d\u7684\u6837\u672c，\u5176STR\u56fe\u8c31\u4e0eHeLa\u3001HEK-293\u3001SH-SY5Y\u7b49\u5df2\u77e5\u7ec6\u80de\u7cfb\u5339\u914d\u5ea6\u226580%，\u5219\u53ef\u76f4\u63a5\u5224\u5b9a\u5176\u4e3a\u7ec6\u80de\u7cfb\u800c\u975e\u539f\u4ee3\u7ec6\u80de<\/p>\n

\u5c40\u9650\u6027：\u4f46\u8fd9\u4e2a\u65b9\u6cd5\u6709\u4e00\u5b9a\u7684\u5c40\u9650\u6027，STR\u5206\u578b\u4f53\u7cfb\u4e3b\u8981\u9002\u7528\u4e8e\u4eba\u6e90\u7ec6\u80de\u53ca\u90e8\u5206\u5c0f\u9f20\u7ec6\u80de，\u5176\u4ed6\u7269\u79cd（\u5982\u5927\u9f20\u3001\u5154\u3001\u732a\u7b49）\u76ee\u524d\u5c1a\u65e0\u6807\u51c6\u5316STR\u6570\u636e\u5e93\u201d<\/p>\n

\u603b\u7ed3：\u65b9\u6cd5\u600e\u4e48\u9009？\u5206\u6b65\u9a8c\u8bc1\u7b56\u7565<\/h3>\n

\u5404\u65b9\u6cd5\u600e\u4e48\u7ec4\u5408？\u63a8\u8350\u4ece\u6613\u5230\u96be\u3001\u5c42\u5c42\u9012\u8fdb\u7684\u56db\u6b65\u8d70\u7b56\u7565：<\/p>\n\n\n\n\n\n\n\n
\n

\u6b65\u9aa4<\/span><\/p>\n<\/td>\n

\n

\u505a\u4ec0\u4e48<\/span><\/p>\n<\/td>\n

\n

\u6210\u672c<\/span><\/p>\n<\/td>\n

\n

\u65f6\u95f4<\/span><\/p>\n<\/td>\n

\n

\u80fd\u786e\u8ba4\u4ec0\u4e48<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u2460<\/span><\/p>\n<\/td>\n

\n

\u663e\u5fae\u955c\u89c2\u5bdf\u5f62\u6001+\u62cd\u7167\u5b58\u6863<\/span><\/p>\n<\/td>\n

\n

\u8fd1\u4e4e\u96f6<\/span><\/p>\n<\/td>\n

\n

\u5373\u65f6<\/span><\/p>\n<\/td>\n

\n

\u521d\u6b65\u5224\u65ad（\u662f\u5426\u5747\u4e00？\u662f\u5426\u5206\u5316？）<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u2461<\/span><\/p>\n<\/td>\n

\n

\u8bb0\u5f55\u500d\u589e\u65f6\u95f4+\u89c2\u5bdf\u63a5\u89e6\u6291\u5236<\/span><\/p>\n<\/td>\n

\n

\u4f4e<\/span><\/p>\n<\/td>\n

\n

3~7\u5929<\/span><\/p>\n<\/td>\n

\n

\u529f\u80fd\u6027\u5224\u65ad（\u957f\u5f97\u5feb\u4e0d\u5feb？\u53e0\u4e0d\u53e0？）<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u2462<\/span><\/p>\n<\/td>\n

\n

\u68c0\u6d4b2~3\u4e2a\u5206\u5316\u6807\u5fd7\u7269（IF\/FACS）<\/span><\/p>\n<\/td>\n

\n

\u4e2d<\/span><\/p>\n<\/td>\n

\n

3-7\u5929<\/span><\/p>\n<\/td>\n

\n

\u8eab\u4efd\u9a8c\u8bc1（\u6807\u5fd7\u7269\u5728\u4e0d\u5728？）<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u2463<\/span><\/p>\n<\/td>\n

\n

STR\u5206\u578b（\u53ea\u9002\u5408\u4eba\u6e90\u7ec6\u80de\u53ca\u90e8\u5206\u5c0f\u9f20\u7ec6\u80de）<\/span><\/p>\n<\/td>\n

\n

\u4e2d\u9ad8<\/span><\/p>\n<\/td>\n

\n

3~7\u5929<\/span><\/p>\n<\/td>\n

\n

\u6700\u7ec8\u5224\u51b3（\u91d1\u6807\u51c6）<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5728\u5b9e\u9645\u5de5\u4f5c\u4e2d，\u5efa\u8bae\u91c7\u7528\u4ece\u6613\u5230\u96be\u3001\u5c42\u5c42\u9012\u8fdb\u7684\u5206\u6b65\u9a8c\u8bc1\u7b56\u7565：<\/span><\/h4>\n

\u7b2c\u4e00\u6b65（\u63a5\u6536\u7ec6\u80de\u65f6）：\u5012\u7f6e\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\u5f62\u6001\u5e76\u62cd\u7167\u5b58\u6863，\u5224\u65ad\u5f62\u6001\u662f\u5426\u7b26\u5408\u8be5\u7ec6\u80de\u7c7b\u578b\u7684\u5178\u578b\u7279\u5f81，\u4ee5\u53ca\u662f\u5426\u5b58\u5728\u5f62\u6001\u5747\u4e00\u5316\u3001\u6838\u8d28\u6bd4\u589e\u9ad8\u7b49\u7ec6\u80de\u7cfb\u7279\u5f81<\/span><\/p>\n

\u7b2c\u4e8c\u6b65（\u57f9\u517b\u8fc7\u7a0b\u4e2d）：\u8bb0\u5f55\u4f20\u4ee3\u6b21\u6570\u548c\u500d\u589e\u65f6\u95f4，\u89c2\u5bdf\u6c47\u5408\u540e\u662f\u5426\u51fa\u73b0\u63a5\u89e6\u6291\u5236（\u539f\u4ee3\u7279\u5f81）\u8fd8\u662f\u5806\u79ef\u751f\u957f（\u7ec6\u80de\u7cfb\u7279\u5f81）<\/span><\/p>\n

\u7b2c\u4e09\u6b65（\u5982\u521d\u6b65\u89c2\u5bdf\u6709\u7591\u95ee）：\u9009\u53d6\u8be5\u7ec6\u80de\u7c7b\u578b\u76842~3\u4e2a\u5173\u952e\u5206\u5316\u6807\u5fd7\u7269\u8fdb\u884c\u514d\u75ab\u8367\u5149\u6216\u6d41\u5f0f\u68c0\u6d4b，\u5224\u5b9a\u5206\u5316\u72b6\u6001<\/span><\/p>\n

\u7b2c\u56db\u6b65（\u4eba\u6e90\u7ec6\u80de\u786e\u8bc1）：\u59d4\u6258STR\u5206\u578b\u68c0\u6d4b，\u4e0e\u6743\u5a01\u6570\u636e\u5e93\u6bd4\u5bf9，\u83b7\u5f97\u6700\u7ec8\u8eab\u4efd\u5224\u5b9a\u00a0<\/span><\/p>\n

\u7ed3\u8bed<\/h2>\n

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