{"id":78856,"date":"2026-02-28T13:32:57","date_gmt":"2026-02-28T05:32:57","guid":{"rendered":"https:\/\/www.wsisp.com\/helps\/78856.html"},"modified":"2026-02-28T13:32:57","modified_gmt":"2026-02-28T05:32:57","slug":"k15q%ef%bc%9bh2n-vkvkvkvkvpptkvqvkvkv-oh","status":"publish","type":"post","link":"https:\/\/www.wsisp.com\/helps\/78856.html","title":{"rendered":"K15Q\uff1bH2N-VKVKVKVKVpPTKVQVKVKV-OH"},"content":{"rendered":"

\u4e00\u3001\u57fa\u672c\u4fe1\u606f<\/h3>\n

\u82f1\u6587\u540d\u79f0：K15Q；K15Q self-assembling peptide；<\/p>\n

\u4e2d\u6587\u540d\u79f0：K15Q \u81ea\u7ec4\u88c5\u591a\u80bd；\u78f7\u9178\u5316\u81ea\u7ec4\u88c5\u591a\u80bdK15Q；\u7f2c\u6c28\u9170-\u8d56\u6c28\u9170-\u7f2c\u6c28\u9170-\u8d56\u6c28\u9170-\u7f2c\u6c28\u9170-\u8d56\u6c28\u9170-\u7f2c\u6c28\u9170-\u8d56\u6c28\u9170-\u78f7\u9178\u82cf\u6c28\u9170-\u82cf\u6c28\u9170-\u8d56\u6c28\u9170-\u7f2c\u6c28\u9170-\u8c37\u6c28\u9170\u80fa\u9170-\u7f2c\u6c28\u9170-\u8d56\u6c28\u9170-\u7f2c\u6c28\u9170-\u8d56\u6c28\u9170-\u7f2c\u6c28\u9170\u591a\u80bd<\/p>\n

\u6c28\u57fa\u9178\u5e8f\u5217：\u7f2c\u6c28\u9178-\u8d56\u6c28\u9178-\u7f2c\u6c28\u9178-\u8d56\u6c28\u9178-\u7f2c\u6c28\u9178-\u8d56\u6c28\u9178-\u7f2c\u6c28\u9178-\u8d56\u6c28\u9178-\u78f7\u9178\u82cf\u6c28\u9178-\u82cf\u6c28\u9178-\u8d56\u6c28\u9178-\u7f2c\u6c28\u9178-\u8c37\u6c28\u9170\u80fa-\u7f2c\u6c28\u9178-\u8d56\u6c28\u9178-\u7f2c\u6c28\u9178-\u8d56\u6c28\u9178-\u7f2c\u6c28\u9178<\/p>\n

\u5355\u5b57\u6bcd\u5e8f\u5217：H\u2082N-VKVKVKVKVpPTKVQVKVKV-OH<\/p>\n

\u4e09\u5b57\u6bcd\u5e8f\u5217：H2N-Val-Lys-Val-Lys-Val-Lys-Val-Lys-Val-DPro-Pro-Thr-Lys-Val-Gln-Val-Lys-Val-Lys-Val-OH<\/p>\n

\u6c28\u57fa\u9178\u957f\u5ea6：18\u80bd<\/p>\n

\u5206\u5b50\u91cf：2230.86 Da<\/p>\n

\u5206\u5b50\u5f0f：C106H196N28O23<\/p>\n

\u7b49\u7535\u70b9：pI\u22488.9（\u5f3a\u78b1\u6027，\u56e0\u542b\u591a\u4e2a\u8d56\u6c28\u9178（Lys）\u6b63\u7535\u8377\u6b8b\u57fa，\u78f7\u9178\u5316\u82cf\u6c28\u9178\u7684\u5fae\u5f31\u8d1f\u7535\u8377\u3001\u8c37\u6c28\u9170\u80fa（Gln）\u7684\u4e2d\u6027\u7279\u5f81，\u4ec5\u8f7b\u5fae\u4e2d\u548c\u90e8\u5206\u6b63\u7535\u8377，\u4e0d\u6539\u53d8\u6574\u4f53\u5f3a\u78b1\u6027）<\/p>\n

\u7ed3\u6784\u5f0f：<\/p>\n

\"\"<\/h3>\n

<\/h3>\n

\u4e8c\u3001\u4f5c\u7528\u673a\u7406<\/h3>\n

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1. \u81ea\u7ec4\u88c5\u542f\u52a8\u4e0e\u53cc\u91cd\u8c03\u63a7：\u5728\u5f31\u78b1\u6027\u81f3\u4e2d\u6027pH\u503c（7.0-8.5）\u53ca\u751f\u7406\u76d0\u79bb\u5b50\u6d53\u5ea6\u6761\u4ef6\u4e0b，K15Q\u5206\u5b50\u95f4\u901a\u8fc7Val\u6b8b\u57fa\u7684\u758f\u6c34\u805a\u96c6\u3001Lys\u207a\u4e0epThr\u78f7\u9178\u57fa\u56e2\u7684\u9759\u7535\u914d\u5bf9\u3001Q\u6b8b\u57fa\u9170\u80fa\u57fa\u5f62\u6210\u7684\u5206\u5b50\u95f4\u6c22\u952e\u53ca\u6574\u4f53\u5206\u5b50\u95f4\u6c22\u952e\u534f\u540c\u4f5c\u7528，\u542f\u52a8\u81ea\u7ec4\u88c5\u8fc7\u7a0b；\u78f7\u9178\u5316\u82cf\u6c28\u9178\u53ef\u7cbe\u51c6\u8c03\u63a7\u5206\u5b50\u95f4\u9759\u7535\u4f5c\u7528\u5f3a\u5ea6，\u5b9e\u73b0\u81ea\u7ec4\u88c5\u901f\u5ea6\u7684\u53ef\u63a7\u8c03\u8282（\u53bb\u78f7\u9178\u5316\u540e\u7ec4\u88c5\u901f\u5ea6\u663e\u8457\u52a0\u5feb），Q\u6b8b\u57fa\u901a\u8fc7\u5f62\u6210\u989d\u5916\u6c22\u952e，\u7a33\u5b9a\u81ea\u7ec4\u88c5\u7ed3\u6784\u3001\u5ef6\u7f13\u89e3\u7ec4\u88c5\u8fdb\u7a0b，\u4e8c\u8005\u534f\u540c\u5b9e\u73b0\u81ea\u7ec4\u88c5\u884c\u4e3a\u7684\u53cc\u91cd\u8c03\u63a7；<\/p>\n

2. \u4eff\u751f\u5fae\u73af\u5883\u6784\u5efa：\u7ec4\u88c5\u5f62\u6210\u7684\u4e09\u7ef4\u6c34\u51dd\u80f6\u7f51\u7edc\u5177\u6709\u9ad8\u542b\u6c34\u91cf（＞90%）\u3001\u9ad8\u5b54\u9699\u7387\u3001\u529b\u5b66\u6027\u80fd\u7a33\u5b9a\u7684\u7279\u70b9，\u5176\u7eb3\u7c73\u7ea4\u7ef4\u7ed3\u6784\u4e0e\u5929\u7136\u7ec6\u80de\u5916\u57fa\u8d28（ECM）\u9ad8\u5ea6\u76f8\u4f3c，Q\u6b8b\u57fa\u7684\u5b58\u5728\u53ef\u589e\u5f3a\u6c34\u51dd\u80f6\u4e0e\u7ec6\u80de\u5916\u57fa\u8d28\u86cb\u767d\u7684\u7ed3\u5408\u80fd\u529b，\u4e3a\u7ec6\u80de\u9ecf\u9644\u3001\u94fa\u5c55\u3001\u589e\u6b96\u53ca\u5206\u5316\u63d0\u4f9b\u7a33\u5b9a\u652f\u6491，\u9002\u914d\u795e\u7ecf\u3001\u8f6f\u9aa8\u3001\u76ae\u80a4\u7b49\u591a\u79cd\u7ec4\u7ec7\u6765\u6e90\u7ec6\u80de\u7684\u4f53\u5916\u57f9\u517b\u4e0e\u4f53\u5185\u79fb\u690d\u9700\u6c42；<\/p>\n

3. \u5206\u5b50\u7ed3\u5408\u4e0e\u9776\u5411\u9012\u9001\u529f\u80fd：K15Q\u7684\u5f3a\u78b1\u6027（\u591a\u4e2aLys\u207a）\u3001\u78f7\u9178\u5316\u4f4d\u70b9（pThr\u8d1f\u7535）\u53caQ\u6b8b\u57fa\u7684\u9170\u80fa\u57fa，\u4f7f\u5176\u53ef\u4e0e\u5e26\u8d1f\u7535\u7684\u751f\u7269\u5206\u5b50（\u5982DNA\u3001RNA\u3001\u86cb\u767d\u3001\u8102\u8d28\u4f53）\u53d1\u751f\u7279\u5f02\u6027\u7ed3\u5408，\u540c\u65f6\u78f7\u9178\u5316\u82cf\u6c28\u9178\u53ef\u4e0e\u7ec6\u80de\u8868\u9762\u78f7\u9178\u9176\u7279\u5f02\u6027\u8bc6\u522b，Q\u6b8b\u57fa\u53ef\u4e0e\u7ec6\u80de\u8868\u9762\u542b\u6c28\u57fa\u3001\u7fa7\u57fa\u7684\u53d7\u4f53\u7ed3\u5408，\u53cc\u91cd\u63d0\u5347\u9776\u5411\u9012\u9001\u6548\u7387，\u51cf\u5c11\u8131\u9776\u6548\u5e94，\u540c\u65f6\u53ef\u4fdd\u62a4\u654f\u611f\u751f\u7269\u5206\u5b50\u514d\u53d7\u964d\u89e3；<\/p>\n

4. \u751f\u7269\u76f8\u5bb9\u6027\u4e0e\u53ef\u964d\u89e3\u6027：\u8be5\u591a\u80bd\u7531\u5929\u7136\u6c28\u57fa\u9178\u7ec4\u6210，\u542b\u751f\u7406\u6027\u78f7\u9178\u5316\u82cf\u6c28\u9178\u4fee\u9970\u4e0eQ\u6b8b\u57fa，\u65e0\u514d\u75ab\u539f\u6027，\u4e0d\u4f1a\u5f15\u53d1\u673a\u4f53\u514d\u75ab\u6392\u65a5\u53cd\u5e94，\u53ef\u88ab\u4f53\u5185\u86cb\u767d\u9176（\u5982\u80f0\u86cb\u767d\u9176\u3001\u78f7\u9178\u9176）\u9010\u6b65\u964d\u89e3，\u78f7\u9178\u5316\u82cf\u6c28\u9178\u53ef\u88ab\u78f7\u9178\u9176\u53bb\u78f7\u9178\u5316，Q\u6b8b\u57fa\u53ef\u88ab\u9170\u80fa\u9176\u964d\u89e3，\u6700\u7ec8\u964d\u89e3\u4e3a\u6c28\u57fa\u9178\u3001\u78f7\u9178\u57fa\u56e2，\u88ab\u673a\u4f53\u4ee3\u8c22\u5229\u7528，\u65e0\u6b8b\u7559\u6bd2\u6027；<\/p>\n

5. \u7279\u5f02\u6027\u5206\u5b50\u8bc6\u522b\u529f\u80fd：Q\u6b8b\u57fa\u7684\u9170\u80fa\u57fa\u53ef\u4e0e\u751f\u7269\u5206\u5b50（\u5982\u86cb\u767d\u3001\u591a\u7cd6）\u7684\u529f\u80fd\u57fa\u56e2\u53d1\u751f\u7279\u5f02\u6027\u6c22\u952e\u7ed3\u5408，\u4f7fK15Q\u6c34\u51dd\u80f6\u5177\u5907\u4e00\u5b9a\u7684\u5206\u5b50\u8bc6\u522b\u80fd\u529b，\u53ef\u7528\u4e8e\u7279\u5f02\u6027\u751f\u7269\u5206\u5b50\u7684\u5305\u57cb\u4e0e\u7f13\u91ca，\u62d3\u5c55\u5176\u5728\u751f\u7269\u4f20\u611f\u3001\u9776\u5411\u9012\u9001\u9886\u57df\u7684\u5e94\u7528\u3002<\/p>\n

\u4e09\u3001\u7814\u7a76\u8fdb\u5c55<\/h3>\n

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\u4e09\u662f\u7ed3\u6784\u4f18\u5316\u4e0e\u590d\u5408\u5e94\u7528，\u76ee\u524d\u7814\u7a76\u591a\u805a\u7126\u4e8eK15Q\u7684\u78f7\u9178\u5316\u4f4d\u70b9\u4fee\u9970（\u5982\u53cc\u78f7\u9178\u5316\u4fee\u9970）\u3001Q\u6b8b\u57fa\u4fee\u9970，\u4ee5\u8c03\u8282\u5176\u81ea\u7ec4\u88c5\u6027\u80fd\u3001\u9776\u5411\u6027\u53ca\u4f53\u5185\u534a\u8870\u671f，\u540c\u65f6\u63a2\u7d22\u5176\u4e0eEAK\u7cfb\u5217\u3001LK\u7cfb\u5217\u3001RADA\u7cfb\u5217\u591a\u80bd\u53ca\u751f\u7269\u6d3b\u6027\u56e0\u5b50（\u5982\u795e\u7ecf\u751f\u957f\u56e0\u5b50\u3001\u78f7\u9178\u9176\u6291\u5236\u5242）\u7684\u534f\u540c\u4f5c\u7528，\u6784\u5efa\u201c\u53ef\u63a7\u7ec4\u88c5+\u9776\u5411\u9012\u9001+\u5206\u5b50\u8bc6\u522b\u201d\u7684\u590d\u5408\u6c34\u51dd\u80f6\u6750\u6599，\u62d3\u5c55\u5176\u5728\u4e34\u5e8a\u524d\u7814\u7a76\u4e2d\u7684\u5e94\u7528\u573a\u666f\u3002\u6574\u4f53\u800c\u8a00，K15Q\u56e0\u81ea\u7ec4\u88c5\u53ef\u63a7\u3001\u9776\u5411\u6027\u5f3a\u3001\u5177\u5907\u5206\u5b50\u8bc6\u522b\u529f\u80fd，\u5df2\u6210\u4e3a\u591a\u529f\u80fd\u751f\u7269\u6750\u6599\u7814\u7a76\u7684\u5e38\u7528\u5e8f\u5217，\u76ee\u524d\u4ecd\u5904\u4e8e\u4e34\u5e8a\u524d\u7814\u7a76\u9636\u6bb5，\u672a\u6765\u91cd\u70b9\u65b9\u5411\u4e3a\u4fee\u9970\u4f4d\u70b9\u4e0eQ\u6b8b\u57fa\u7684\u7cbe\u51c6\u8c03\u63a7\u3001\u89c4\u6a21\u5316\u5236\u5907\u53ca\u4e34\u5e8a\u8f6c\u5316\u5e94\u7528，\u5c24\u5176\u5728\u795e\u7ecf\u7ec4\u7ec7\u518d\u751f\u3001\u7279\u5f02\u6027\u9776\u5411\u6cbb\u7597\u7b49\u9886\u57df\u5177\u6709\u72ec\u7279\u4f18\u52bf\u4e0e\u5e7f\u9614\u524d\u666f\u3002<\/p>\n

\u56db\u3001\u6eb6\u89e3\u4e0e\u4fdd\u5b58<\/h3>\n

\u6eb6\u89e3\u7279\u6027：K15Q\u4e3a\u5f3a\u78b1\u6027\u78f7\u9178\u531618\u80bd，\u542b\u758f\u6c34\u6027Val\u6b8b\u57fa\u4e0eQ\u6b8b\u57fa，\u6613\u6eb6\u4e8e\u8d85\u7eaf\u6c34\u3001\u7a00\u76d0\u9178\u6eb6\u6db2（0.1%-1%）\u53caPBS\u7f13\u51b2\u6db2（pH7.4），\u6eb6\u89e3\u65f6\u9700\u8fdb\u884c\u4f4e\u529f\u7387\u8d85\u58f0\u8f85\u52a9\u6eb6\u89e3（50-100W，5-10\u5206\u949f），\u5e38\u6e29\u7f13\u6162\u6405\u62cc，\u907f\u514d\u9ad8\u6e29（＞30\u2103）\u5bfc\u81f4\u591a\u80bd\u53d8\u6027\u3001\u78f7\u9178\u5316\u82cf\u6c28\u9178\u4f4d\u70b9\u8131\u843d；\u5728\u9ad8\u76d0\u6d53\u5ea6（＞0.5M NaCl）\u6216\u5f3a\u78b1\u6027\u73af\u5883（pH＞10.0）\u4e0b，\u6613\u53d1\u751f\u805a\u96c6，\u4e14\u53ef\u80fd\u5bfc\u81f4\u78f7\u9178\u5316\u4f4d\u70b9\u4e0d\u7a33\u5b9a，\u9700\u907f\u514d\u4e0a\u8ff0\u6781\u7aef\u73af\u5883；\u6eb6\u89e3\u540e\u9700\u5c3d\u5feb\u4f7f\u7528，\u907f\u514d\u957f\u65f6\u95f4\u653e\u7f6e\u5bfc\u81f4\u78f7\u9178\u5316\u4f4d\u70b9\u6c34\u89e3\u53ca\u591a\u80bd\u805a\u96c6\u3002<\/p>\n

\u4fdd\u5b58\u6761\u4ef6：\u7c89\u672b\u72b6\u6001\u4e0b，\u4e8e-20\u2103\u5e72\u71e5\u907f\u5149\u73af\u5883\u4e2d\u5bc6\u5c01\u4fdd\u5b58，\u53ef\u7a33\u5b9a\u5b58\u653e12\u4e2a\u6708\u4ee5\u4e0a，\u9700\u9694\u7edd\u7a7a\u6c14\u3001\u6e7f\u6c14\u4e0e\u6c27\u5316\u5242，\u9632\u6b62\u78f7\u9178\u5316\u82cf\u6c28\u9178\u4f4d\u70b9\u6c34\u89e3\u3001\u591a\u80bd\u6c27\u5316\u53caQ\u6b8b\u57fa\u53d8\u6027；\u6c34\u6eb6\u6db2\u9700\u4f4e\u6e29（4\u2103）\u77ed\u671f\u6682\u5b58，\u5b58\u653e\u65f6\u95f4\u4e0d\u8d85\u8fc748\u5c0f\u65f6，\u5efa\u8bae\u5206\u88c5\u4fdd\u5b58（\u6bcf\u7ba10.5-1mL），\u907f\u514d\u53cd\u590d\u51bb\u878d；\u957f\u671f\u4fdd\u5b58\u7684\u6c34\u6eb6\u6db2\u9700\u6dfb\u52a0\u9002\u91cf\u9632\u8150\u5242（\u59820.01%\u53e0\u6c2e\u94a0）\u53ca\u78f7\u9178\u9176\u6291\u5236\u5242（\u5982Na\u2083VO\u2084），\u9632\u6b62\u5fae\u751f\u7269\u6c61\u67d3\u53ca\u78f7\u9178\u5316\u82cf\u6c28\u9178\u4f4d\u70b9\u53bb\u78f7\u9178\u5316；\u907f\u514d\u5f3a\u5149\u7167\u5c04\u53ca\u9ad8\u6e29（＞25\u2103）\u73af\u5883，\u9632\u6b62\u591a\u80bd\u964d\u89e3\u3001\u53d8\u6027\u3001\u78f7\u9178\u5316\u4f4d\u70b9\u8131\u843d\u6216\u81ea\u7ec4\u88c5\u7ed3\u6784\u5f02\u5e38\u3002<\/p>\n

\u4e94\u3001\u76f8\u5173\u591a\u80bd<\/h3>\n

\u540c\u5c5e\u78f7\u9178\u5316\u81ea\u7ec4\u88c5\u591a\u80bd\u53ca\u79bb\u5b50\u4e92\u8865\u578b\u591a\u80bd\u4f53\u7cfb，\u4e14\u4e0eK15Q\u5e8f\u5217\u76f8\u4f3c\u6216\u529f\u80fd\u76f8\u8fd1\u7684\u6838\u5fc3\u76f8\u5173\u591a\u80bd\u5305\u62ec：MAX1（VKVKVKVKVpPTKVEVKVKV，19\u80bd，pThr\u4fee\u9970，\u542bGlu\u6b8b\u57fa\u3001\u65e0Q\u6b8b\u57fa）\u3001K15E（\u5e8f\u5217\u4e0eK15Q\u76f8\u8fd1，Q\u6b8b\u57fa\u66ff\u6362\u4e3aGlu\u6b8b\u57fa，\u65e0Q\u7279\u5f81）\u3001KV18（VKVKVKVKVKVKVKVKVKV，18\u80bd，\u65e0\u78f7\u9178\u5316\u4fee\u9970\u4e0eQ\u6b8b\u57fa，K15Q\u7684\u672a\u4fee\u9970\u7c7b\u4f3c\u7269）\u3001EAK16-I（AEAEAKAKAEAEAKAK，16\u80bd，\u79bb\u5b50\u4e92\u8865\u578b，\u65e0\u78f7\u9178\u5316\u4fee\u9970\u4e0eQ\u6b8b\u57fa）\u3001LK13（LKLKLKLKLKLKL，13\u80bd，\u5f3a\u78b1\u6027，\u65e0\u78f7\u9178\u5316\u4fee\u9970\u4e0eQ\u6b8b\u57fa）\u3001pKV-Q（\u542bQ\u6b8b\u57fa\u7684\u78f7\u9178\u5316KV\u77ed\u80bd，10-15\u80bd，K15Q\u6838\u5fc3\u5e8f\u5217\u622a\u77ed\u4f53）\u3002\u6b64\u7c7b\u591a\u80bd\u4e2d，K15E\u3001KV18\u4e0eK15Q\u4eb2\u7f18\u6027\u6700\u9ad8，\u5747\u4e3aKV\u4ea4\u66ff\u5e8f\u5217，\u4ec5\u5728\u7279\u5f81\u6b8b\u57fa\u3001\u78f7\u9178\u5316\u4fee\u9970\u4e0a\u5b58\u5728\u5dee\u5f02；K15Q\u76f8\u8f83\u4e8eMAX1，\u4ee5Q\u6b8b\u57fa\u66ff\u4ee3Glu\u6b8b\u57fa，\u5206\u5b50\u7ed3\u5408\u80fd\u529b\u4e0e\u7ed3\u6784\u7a33\u5b9a\u6027\u66f4\u4f18，\u76f8\u8f83\u4e8e\u65e0Q\u6b8b\u57fa\u7684\u591a\u80bd，\u5177\u5907\u72ec\u7279\u7684\u5206\u5b50\u8bc6\u522b\u529f\u80fd，\u53ef\u6839\u636e\u5177\u4f53\u7814\u7a76\u9700\u6c42（\u5982\u7ec4\u88c5\u8c03\u63a7\u3001\u9776\u5411\u9012\u9001\u3001\u5206\u5b50\u8bc6\u522b\u3001\u7ec4\u7ec7\u9002\u914d\u6027）\u9009\u62e9\u4f7f\u7528\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u4e00\u3001\u57fa\u672c\u4fe1\u606f
\n\u82f1\u6587\u540d\u79f0：K15Q；K15Q self-assembling peptide；
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\n\u6c28\u57fa\u9178\u5e8f\u5217：\u7f2c\u6c28\u9178-\u8d56\u6c28\u9178-\u7f2c\u6c28\u9178-\u8d56\u6c28\u9178-\u7f2c\u6c28\u9178-\u8d56\u6c28\u9178-\u7f2c\u6c28\u9178-<\/p>\n","protected":false},"author":2,"featured_media":78855,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[305],"topic":[],"class_list":["post-78856","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-server","tag-305"],"yoast_head":"\nK15Q\uff1bH2N-VKVKVKVKVpPTKVQVKVKV-OH - \u7f51\u7855\u4e92\u8054\u5e2e\u52a9\u4e2d\u5fc3<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.wsisp.com\/helps\/78856.html\" \/>\n<meta property=\"og:locale\" content=\"zh_CN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"K15Q\uff1bH2N-VKVKVKVKVpPTKVQVKVKV-OH - 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