{"id":62645,"date":"2026-01-20T13:18:50","date_gmt":"2026-01-20T05:18:50","guid":{"rendered":"https:\/\/www.wsisp.com\/helps\/62645.html"},"modified":"2026-01-20T13:18:50","modified_gmt":"2026-01-20T05:18:50","slug":"chip-cut%e7%bb%93%e6%9e%9c%e8%a7%a3%e8%af%bb%e4%b9%8bmotif%e5%88%86%e6%9e%90%ef%bc%8c%e5%85%85%e5%88%86%e5%88%a9%e7%94%a8%e7%bb%84%e5%ad%a6%e6%95%b0%e6%8d%ae","status":"publish","type":"post","link":"https:\/\/www.wsisp.com\/helps\/62645.html","title":{"rendered":"ChIP\/CUT\u7ed3\u679c\u89e3\u8bfb\u4e4bMotif\u5206\u6790\uff0c\u5145\u5206\u5229\u7528\u7ec4\u5b66\u6570\u636e"},"content":{"rendered":"

ChIP-seq（\u67d3\u8272\u8d28\u514d\u75ab\u6c89\u6dc0\u6d4b\u5e8f）\u548c\u8fd1\u5e74\u6765\u5927\u706b\u7684CUT&Tag（\u9776\u5411\u526a\u5207\u4e0e\u6807\u8bb0\u6280\u672f）\u662f\u8868\u89c2\u9057\u4f20\u5b66\u7814\u7a76\u7684\u201c\u9ec4\u91d1\u5de5\u5177\u201d，\u6838\u5fc3\u4f5c\u7528\u662f\u7cbe\u51c6\u5b9a\u4f4d\u86cb\u767d\u8d28（\u5982\u8f6c\u5f55\u56e0\u5b50\u3001\u7ec4\u86cb\u767d\u4fee\u9970）\u5728\u57fa\u56e0\u7ec4\u4e0a\u7684\u7ed3\u5408\u4f4d\u70b9\u3002\u800c\u5728ChIP\/CUT\u7ed3\u679c\u89e3\u8bfb\u4e2d，Motif\u5206\u6790\u7edd\u5bf9\u662f\u7ed5\u4e0d\u5f00\u7684\u6838\u5fc3\u73af\u8282，\u5b83\u80fd\u5e2e\u4f60\u4ece\u6d77\u91cf\u7ed3\u5408\u4f4d\u70b9\u4e2d，\u6316\u6398\u51fa\u86cb\u767d\u8d28\u8bc6\u522b\u7ed3\u5408\u7684\u6838\u5fc3DNA\u5e8f\u5217\u57fa\u5e8f，\u8fdb\u800c\u63ed\u793a\u8f6c\u5f55\u8c03\u63a7\u7684\u5206\u5b50\u673a\u5236\u3002<\/p>\n<\/p>\n

\u4e00\u3001\u4ec0\u4e48\u662fMotif？\u4e3a\u4ec0\u4e48ChIP\/CUT\u5fc5\u987b\u505aMotif\u5206\u6790？<\/h2>\n<\/p>\n

（\u4e00）Motif\u7684\u6838\u5fc3\u5b9a\u4e49：\u86cb\u767d\u8d28\u8bc6\u522b\u7684\u201cDNA\u5bc6\u7801\u201d<\/p>\n

Motif（\u57fa\u5e8f），\u7279\u6307\u4e00\u6bb5\u5177\u6709\u9ad8\u5ea6\u4fdd\u5b88\u6027\u7684\u77edDNA\u5e8f\u5217（\u901a\u5e386-20 bp），\u662f\u8f6c\u5f55\u56e0\u5b50\u3001\u7ec4\u86cb\u767d\u4fee\u9970\u76f8\u5173\u86cb\u767d\u7b49DNA\u7ed3\u5408\u86cb\u767d\u7684\u6838\u5fc3\u8bc6\u522b\u4f4d\u70b9\u3002\u7b80\u5355\u6765\u8bf4，Motif\u5c31\u662fDNA\u4e0a\u7684\u201c\u4e13\u5c5e\u63a5\u5934\u201d，\u800cDNA\u7ed3\u5408\u86cb\u767d\u5c31\u662f\u5bf9\u5e94\u7684\u201c\u8fde\u63a5\u5668\u201d，\u53ea\u6709\u4e24\u8005\u7cbe\u51c6\u5339\u914d，\u86cb\u767d\u8d28\u624d\u80fd\u7ed3\u5408\u5230DNA\u4e0a\u53d1\u6325\u8c03\u63a7\u4f5c\u7528\u3002<\/p>\n

\u6bd4\u5982\u5927\u5bb6\u719f\u77e5\u7684p53\u86cb\u767d，\u5176\u8bc6\u522b\u7684Motif\u662f\u4e00\u6bb510 bp\u7684\u4fdd\u5b88\u5e8f\u5217\u201cRRRCWWGYYY\u201d（R=A\/G，W=A\/T，Y=C\/T）；\u518d\u6bd4\u5982\u8f6c\u5f55\u56e0\u5b50NF-\u03baB，\u5176\u6838\u5fc3Motif\u4e3a\u201cGGGRNNYYCC\u201d（R=A\/G，N=\u4efb\u610f\u78b1\u57fa，Y=C\/T）\u3002\u8fd9\u4e9b\u4fdd\u5b88\u5e8f\u5217\u7684\u5b58\u5728，\u662f\u86cb\u767d\u8d28\u7279\u5f02\u6027\u7ed3\u5408\u57fa\u56e0\u7ec4\u7684\u5173\u952e\u57fa\u7840\u3002<\/p>\n

\u9700\u8981\u6ce8\u610f\u7684\u662f，Motif\u7684\u4fdd\u5b88\u6027\u5e76\u975e100%（\u5b8c\u5168\u4fdd\u5b88\u7684\u5e8f\u5217\u6781\u5c11），\u66f4\u591a\u662f\u201c\u76f8\u5bf9\u4fdd\u5b88\u201d，\u5373\u6838\u5fc3\u78b1\u57fa\u4f4d\u7f6e\u9ad8\u5ea6\u56fa\u5b9a，\u975e\u6838\u5fc3\u4f4d\u7f6e\u5141\u8bb8\u5c11\u91cf\u78b1\u57fa\u53d8\u5f02\u3002\u8fd9\u79cd\u7279\u6027\u4e5f\u51b3\u5b9a\u4e86Motif\u5206\u6790\u9700\u8981\u901a\u8fc7\u751f\u7269\u4fe1\u606f\u5b66\u65b9\u6cd5，\u4ece\u5927\u91cf\u5e8f\u5217\u4e2d\u6316\u6398\u51fa\u9690\u85cf\u7684\u4fdd\u5b88\u89c4\u5f8b\u3002<\/p>\n

（\u4e8c）Motif\u5206\u6790\u4e0eChIP\/CUT\u7684\u6838\u5fc3\u5173\u8054：\u4ece\u201c\u4f4d\u70b9\u201d\u5230\u201c\u673a\u5236\u201d\u7684\u6865\u6881<\/p>\n

\u6211\u4eec\u505aChIP\/CUT\u5b9e\u9a8c，\u6700\u7ec8\u4f1a\u5f97\u5230\u4e00\u6279\u201c\u86cb\u767d\u8d28\u7ed3\u5408\u5cf0\u201d（Peak），\u4e5f\u5c31\u662f\u86cb\u767d\u8d28\u5728\u57fa\u56e0\u7ec4\u4e0a\u7684\u7ed3\u5408\u533a\u57df\u3002\u4f46\u77e5\u9053\u201c\u7ed3\u5408\u5728\u54ea\u91cc\u201d\u53ea\u662f\u7b2c\u4e00\u6b65，\u66f4\u91cd\u8981\u7684\u662f\u641e\u6e05\u695a\u201c\u4e3a\u4ec0\u4e48\u7ed3\u5408\u5728\u8fd9\u91cc\u201d\u201c\u7ed3\u5408\u540e\u8c03\u63a7\u4e86\u54ea\u4e9b\u57fa\u56e0\u201d\u3002\u800cMotif\u5206\u6790，\u6b63\u662f\u8fde\u63a5\u201c\u7ed3\u5408\u4f4d\u70b9\u201d\u4e0e\u201c\u8c03\u63a7\u673a\u5236\u201d\u7684\u6838\u5fc3\u6865\u6881\u3002\u5177\u4f53\u6765\u8bf4，ChIP\/CUT\u7ed3\u679c\u4e2d\u7684Motif\u5206\u6790，\u4e3b\u8981\u4ef7\u503c\u4f53\u73b0\u57283\u4e2a\u65b9\u9762：<\/p>\n

（1）\u9a8c\u8bc1\u5b9e\u9a8c\u53ef\u9760\u6027：\u5982\u679c\u6211\u4eec\u9488\u5bf9\u67d0\u4e00\u5df2\u77e5\u8f6c\u5f55\u56e0\u5b50（\u5982MYC）\u505aChIP\/CUT，Motif\u5206\u6790\u82e5\u80fd\u663e\u8457\u5bcc\u96c6\u5230MYC\u7684\u5df2\u77e5Motif，\u5c31\u8bf4\u660e\u5b9e\u9a8c\u7ed3\u679c\u53ef\u9760，\u7ed3\u5408\u5cf0\u662f\u771f\u5b9e\u7684，\u800c\u975e\u80cc\u666f\u566a\u97f3；\u53cd\u4e4b，\u82e5\u672a\u5bcc\u96c6\u5230\u76ee\u6807Motif，\u5219\u53ef\u80fd\u63d0\u793a\u5b9e\u9a8c\u5b58\u5728\u95ee\u9898（\u5982\u6297\u4f53\u7279\u5f02\u6027\u5dee\u3001\u5b9e\u9a8c\u6c61\u67d3\u3001\u5cf0\u8bc6\u522b\u9608\u503c\u4e0d\u5f53\u7b49）\u3002<\/p>\n

（2）\u6316\u6398\u6838\u5fc3\u7ed3\u5408\u57fa\u5e8f：\u5bf9\u4e8e\u672a\u77e5\u7ed3\u5408\u5e8f\u00b7\u5217\u7684\u86cb\u767d\u8d28（\u5982\u65b0\u578b\u8f6c\u5f55\u56e0\u5b50\u3001\u672a\u77e5\u529f\u80fd\u7684\u7ec4\u86cb\u767d\u4fee\u9970\u76f8\u5173\u86cb\u767d），Motif\u5206\u6790\u80fd\u4ece\u7ed3\u5408\u5cf0\u5e8f\u5217\u4e2d，\u4ece\u5934\u9884\u6d4b\u51fa\u5176\u53ef\u80fd\u8bc6\u522b\u7684\u4fdd\u5b88Motif，\u4e3a\u540e\u7eed\u529f\u80fd\u9a8c\u8bc1\u63d0\u4f9b\u6838\u5fc3\u9776\u70b9\u3002<\/p>\n

（3）\u63ed\u793a\u8c03\u63a7\u7f51\u7edc：\u5355\u4e2aMotif\u53ef\u80fd\u5bf9\u5e94\u591a\u79cd\u86cb\u767d\u8d28，\u6216\u5355\u4e2a\u86cb\u767d\u8d28\u53ef\u80fd\u8bc6\u522b\u591a\u79cdMotif；\u540c\u65f6，Motif\u7684\u5bcc\u96c6\u5f80\u5f80\u4e0e\u7279\u5b9a\u751f\u7269\u5b66\u8fc7\u7a0b（\u5982\u7ec6\u80de\u589e\u6b96\u3001\u5206\u5316\u3001\u51cb\u4ea1）\u76f8\u5173\u3002\u901a\u8fc7Motif\u5206\u6790，\u53ef\u63a8\u6d4b\u76ee\u6807\u86cb\u767d\u8d28\u7684\u534f\u540c\u8c03\u63a7\u56e0\u5b50，\u8fdb\u800c\u6784\u5efa\u8f6c\u5f55\u8c03\u63a7\u7f51\u7edc\u3002<\/p>\n

（\u4e09）\u5e38\u89c1Motif\u7c7b\u578b<\/p>\n

\u5728ChIP\/CUT\u7684Motif\u5206\u6790\u4e2d，\u5e38\u89c1\u7684Motif\u7c7b\u578b\u53ef\u4ee5\u5927\u81f4\u5206\u4e3a3\u7c7b：<\/p>\n

（1）\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408Motif（TF Motif）：\u8fd9\u662f\u6700\u5e38\u89c1\u7684\u7c7b\u578b，\u5bf9\u5e94\u8f6c\u5f55\u56e0\u5b50（\u5982\u6fc0\u6d3b\u5b50\u3001\u6291\u5236\u5b50）\u8bc6\u522b\u7684DNA\u5e8f\u5217\u3002\u8fd9\u7c7bMotif\u901a\u5e38\u4f4d\u4e8e\u57fa\u56e0\u542f\u52a8\u5b50\u3001\u589e\u5f3a\u5b50\u533a\u57df，\u76f4\u63a5\u53c2\u4e0e\u57fa\u56e0\u8f6c\u5f55\u7684\u8c03\u63a7\u3002<\/p>\n

（2）\u7ec4\u86cb\u767d\u4fee\u9970\u76f8\u5173Motif：\u90e8\u5206\u7ec4\u86cb\u767d\u4fee\u9970（\u5982H3K4me3\u3001H3K27ac）\u7684\u7ed3\u5408\u533a\u57df，\u4f1a\u4f34\u968f\u7279\u5b9a\u7684DNA\u5e8f\u5217\u57fa\u5e8f\u5bcc\u96c6\u3002\u8fd9\u7c7bMotif\u53ef\u80fd\u4e0e\u7ec4\u86cb\u767d\u4fee\u9970\u9176\u7684\u8bc6\u522b\u76f8\u5173，\u6216\u4e0e\u8be5\u4fee\u9970\u8c03\u63a7\u7684\u8f6c\u5f55\u8fc7\u7a0b\u76f8\u5173\u3002<\/p>\n

（3）\u5176\u4ed6\u529f\u80fdMotif：\u5982\u67d3\u8272\u8d28\u5f00\u653e\u533a\u57df\u76f8\u5173\u7684Motif\u3001DNA\u7532\u57fa\u5316\u76f8\u5173\u7684Motif\u7b49\u3002\u8fd9\u7c7bMotif\u901a\u5e38\u4e0e\u67d3\u8272\u8d28\u7ed3\u6784\u8c03\u63a7\u76f8\u5173\u3002<\/p>\n<\/p>\n

\u4e8c\u3001\u6838\u5fc3\u539f\u7406：Motif\u5206\u6790\u662f\u5982\u4f55\u201c\u627e\u5230\u201d\u4fdd\u5b88\u5e8f\u5217\u7684？<\/h2>\n<\/p>\n

Motif\u5206\u6790\u7684\u5177\u4f53\u64cd\u4f5c\u662f\u63d0\u53d6peaks\u6240\u5728\u533a\u95f4\u7684\u5e8f\u5217，\u901a\u8fc7homer\u7b49\u5bf9peak\u4e4b\u95f4\u5171\u6709\u7684motif\u8fdb\u884c\u626b\u63cf，\u67e5\u627e\u5176\u5171\u6709\u7684motif\u533a\u57df，\u5e76\u7ed8\u5236motif\u56fe\u3002\u4f1a\u6839\u636emotif\u78b1\u57fa\u662f\u5426\u5728\u6570\u636e\u5e93\u4e2d\u6709\u5339\u914d\u5206\u4e3aKnown motif\u548cDe novo Motif\u7684\u8868\u683c，\u4e00\u822c\u5173\u6ce8Known motif\u4e2d\u7684\u4fe1\u606f\u3002<\/p>\n

（\u4e00）\u4ece\u5934\u9884\u6d4b（De novo Motif Discovery）<\/p>\n

\u4ece\u5934\u9884\u6d4b\u7684\u6838\u5fc3\u662f\u201c\u65e0\u5148\u9a8c\u4fe1\u606f\u201d，\u5c31\u662f\u4e0d\u4f9d\u8d56\u5df2\u6709\u7684Motif\u6570\u636e\u5e93，\u76f4\u63a5\u4ece\u5b9e\u9a8c\u5f97\u5230\u7684\u7ed3\u5408\u5cf0\u5e8f\u5217\u4e2d，\u901a\u8fc7\u7b97\u6cd5\u7b5b\u9009\u51fa\u4fdd\u5b88\u5e8f\u5217\u3002\u8fd9\u79cd\u65b9\u6cd5\u9002\u5408\u7814\u7a76\u672a\u77e5\u529f\u80fd\u7684\u86cb\u767d\u8d28，\u6216\u63a2\u7d22\u76ee\u6807\u86cb\u767d\u8d28\u7684\u65b0\u7ed3\u5408\u57fa\u5e8f\u3002\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408\u4f4d\u70b9\u8868\u73b0\u51fa\u4e00\u5b9a\u7684\u5e8f\u5217\u53d8\u5f02\u6027，\u4ee5JASPAR\u8fd9\u4e2a\u6570\u636e\u5e93\u4e3a\u4f8b，JASPAR\u662f\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408\u4f4d\u70b9\u4fe1\u606f\u6570\u636e\u5e93，\u4ee5position frequency matrices（PFMs）\u548cTF flexible models（TFFMs）\u7684\u5f62\u5f0f\u8bb0\u5f55\u4e86\u8f6c\u5f55\u56e0\u5b50\u7684DNA\u7ed3\u5408\u504f\u597d\u4fe1\u606f，\u8fd9\u4e9b\u4fe1\u606f\u53ef\u4ee5\u8f6c\u6362\u4e3a\u4f4d\u7f6e\u6743\u91cd\u77e9\u9635\u3002\u800c\u6211\u4eec\u901a\u8fc7ChIP\/CUT，\u5bf9Peak\u533a\u57df\u9274\u5b9amotif\u5e8f\u5217，\u5728\u5e8f\u5217\u7247\u6bb5\u7684\u6bcf\u4e2a\u4f4d\u7f6e\u4e0a，\u5f97\u5230\u4e0d\u540c\u78b1\u57fa\u7684\u6570\u91cf，\u5f62\u6210\u4e00\u4e2a\u77e9\u9635，\u5c06\u5f97\u5230\u7684motif\u5e8f\u5217\u4e0e\u6570\u636e\u5e93\u8fdb\u884c\u6bd4\u5bf9，\u6839\u636e\u78b1\u57fa\u6570\u91cf\u6743\u91cd，\u5f62\u6210logo\u56fe，\u5b57\u6bcd\u8d8a\u5927\u7684，\u8bf4\u660e\u8fd9\u4e2a\u4f4d\u7f6e\u662f\u8fd9\u4e2a\u78b1\u57fa\u7684\u53ef\u80fd\u6027\u66f4\u5927，\u4ece\u800c\u9274\u5b9a\u51fa\u9776\u86cb\u767dbinding\u7684motif\u3002<\/p>\n

（\u4e8c）\u5df2\u77e5Motif\u5bcc\u96c6\u5206\u6790（Known Motif Enrichment）<\/p>\n

\u5df2\u77e5Motif\u5bcc\u96c6\u5206\u6790\u7684\u6838\u5fc3\u662f\u201c\u6709\u5148\u9a8c\u4fe1\u606f\u201d，\u5c31\u662f\u57fa\u4e8e\u5df2\u6709\u7684Motif\u6570\u636e\u5e93，\u68c0\u9a8c\u5b9e\u9a8c\u5f97\u5230\u7684\u7ed3\u5408\u5cf0\u5e8f\u5217\u4e2d，\u662f\u5426\u663e\u8457\u5bcc\u96c6\u67d0\u7c7b\u5df2\u77e5\u7684Motif\u3002\u8fd9\u79cd\u65b9\u6cd5\u9002\u5408\u7814\u7a76\u5df2\u77e5\u529f\u80fd\u7684\u86cb\u767d\u8d28（\u5982\u7ecf\u5178\u8f6c\u5f55\u56e0\u5b50）\u3002<\/p>\n<\/p>\n

\u4e09\u3001\u5e38\u89c1Motif\u7ed3\u679c\u5c55\u793a\u65b9\u5f0f<\/h2>\n<\/p>\n

（\u4e00）\u62a5\u544a\u4e2dMotif\u5206\u6790\u8868\u683c\u7ed3\u679c\u89e3\u8bfb<\/p>\n<\/p>\n

\"image.png\"<\/p>\n

\u2460 Name：\u8bc6\u522b\u8fd9\u4e2amotif\u7684\u8f6c\u5f55\u56e0\u5b50\u540d\u79f0；<\/p>\n

\u2461 P-Value：\u663e\u8457\u6027\u3002<\/p>\n

\u2462 Target sequences：\u7528\u8f93\u5165\u7684peaks\u627e\u76ee\u7684\u86cb\u767d\u7ed3\u5408\u7684\u89c4\u5f8b\u6027motif，\u5c06\u5bcc\u96c6\u6700\u663e\u8457\u7684peaks\u6216\u603bpeaks\u8f93\u5165homer\u4f5c\u4e3a\u603b\u9776\u5e8f\u5217；<\/p>\n

\u2463 ％ of targets sequences with motif ：\u603b\u9776\u5e8f\u5217\u4e2d\u5305\u542b\u6b64motif\u7684\u5e8f\u5217\u6570\u76ee\u5360\u603b\u9776\u5e8f\u5217\u7684\u767e\u5206\u6bd4；<\/p>\n

\u2464 Background sequences ：\u5e76\u4f1a\u968f\u673a\u5339\u914d\u4e00\u4e9bBackgroud\u5e8f\u5217，\u6839\u636e\u5e8f\u5217\u7279\u5f81\u6311\u9009\u4e00\u4e9b\u5bf9\u5e94\u5e8f\u5217，\u505a\u76f8\u4f3c\u6027motif\u5206\u6790，\u4ee5\u6b64\u5224\u65ad\u6240\u627e\u5230\u7684motif\u662f\u5426\u4e3a\u5047\u9633\u6027\u4fe1\u606f；<\/p>\n

\u2465 ％ of background sequences with motif：\u80cc\u666f\u5e8f\u5217\u4e2d\u5305\u542b\u6b64motif\u7684\u5e8f\u5217\u6570\u76ee\u5360\u603b\u9776\u5e8f\u5217\u7684\u767e\u5206\u6bd4\u3002\u8be5\u503c\u8fc7\u9ad8\u4e3a\u5047\u9633\u6027；<\/p>\n

（\u4e8c）Logo\u5c55\u793a\u56fe：\u76f4\u89c2\u53ef\u89c6\u5316\u7684\u78b1\u57fa\u504f\u597d\u6027<\/p>\n

Logo\u5c55\u793a\u56fe\u662fMotif\u5206\u6790\u4e2d\u6700\u4e3b\u6d41\u3001\u6700\u76f4\u89c2\u7684\u53ef\u89c6\u5316\u5f62\u5f0f，\u6838\u5fc3\u4f5c\u7528\u662f\u5c06Motif\u4e2d\u6bcf\u4e2a\u4f4d\u7f6e\u7684\u78b1\u57fa\u504f\u597d\u6027\u4ee5\u56fe\u5f62\u5316\u65b9\u5f0f\u5448\u73b0，\u8ba9\u7814\u7a76\u8005\u4e00\u773c\u5c31\u80fd\u6355\u6349\u5230\u5173\u952e\u4fdd\u5b88\u533a\u57df，\u7279\u522b\u9002\u5408\u7528\u4e8e\u5feb\u901f\u5224\u65adMotif\u7684\u6838\u5fc3\u7279\u5f81，\u6bd4\u5982\u8bc6\u522b\u4fdd\u5b88\u6027\u6700\u5f3a\u7684\u5173\u952e\u4f4d\u70b9\u3001\u89c2\u5bdf\u78b1\u57fa\u5206\u5e03\u7684\u5bf9\u79f0\u6027（\u5982\u90e8\u5206\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408\u4f4d\u70b9\u5448\u56de\u6587\u7ed3\u6784）\u7b49\u3002\u5728ChIP\/CUT\u7ed3\u679c\u89e3\u8bfb\u4e2d，\u901a\u8fc7\u5bf9\u6bd4\u4e0d\u540c\u6837\u672c\u7684Motif Logo\u56fe，\u8fd8\u80fd\u76f4\u89c2\u53d1\u73b0\u7ec4\u95f4\u5dee\u5f02，\u82e5\u5904\u7406\u7ec4\u67d0Motif\u7684\u5173\u952e\u4fdd\u5b88\u4f4d\u70b9\u5b57\u6bcd\u9ad8\u5ea6\u964d\u4f4e，\u53ef\u80fd\u63d0\u793a\u8be5\u8f6c\u5f55\u56e0\u5b50\u7684\u7ed3\u5408\u7279\u5f02\u6027\u51cf\u5f31\u3002<\/p>\n<\/p>\n

\"\u56fe\u72472.png\"<\/p>\n

\u56fe Homo sapiens GATA1 Motif\u5e8f\u5217<\/p>\n<\/p>\n

Logo\u56fe\u7684\u7eb5\u8f74\u4ee3\u8868\u78b1\u57fa\u7684\u4fdd\u5b88\u6027，，\u5e38\u4ee5bits\u4e3a\u5355\u4f4d，\u6570\u503c\u8d8a\u9ad8\u8868\u793a\u8be5\u4f4d\u7f6e\u7684\u78b1\u57fa\u8d8a\u4fdd\u5b88，\u5728\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408\u7b49\u751f\u7269\u5b66\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u8d8a\u5173\u952e；\u6a2a\u8f74\u5219\u5bf9\u5e94Motif\u7684\u5e8f\u5217\u4f4d\u7f6e，\u4ece5\u2019\u7aef\u52303\u2019\u7aef\u4f9d\u6b21\u6392\u5217\u3002\u56fe\u4e2d\u7684\u6bcf\u4e2a\u5b57\u6bcd（A\u3001T\u3001C\u3001G）\u4ee3\u8868\u5bf9\u5e94\u4f4d\u7f6e\u53ef\u80fd\u51fa\u73b0\u7684\u78b1\u57fa，\u5b57\u6bcd\u7684\u9ad8\u5ea6\u76f4\u63a5\u53cd\u6620\u8be5\u78b1\u57fa\u5728\u8be5\u4f4d\u7f6e\u7684\u51fa\u73b0\u9891\u7387，\u9ad8\u5ea6\u8d8a\u9ad8，\u51fa\u73b0\u9891\u7387\u8d8a\u9ad8，\u504f\u597d\u6027\u8d8a\u5f3a\u3002\u4f8b\u5982，\u82e5\u67d0\u4f4d\u7f6eG\u7684\u5b57\u6bcd\u9ad8\u5ea6\u663e\u8457\u9ad8\u4e8e\u5176\u4ed6\u78b1\u57fa，\u8bf4\u660e\u8be5\u4f4d\u7f6e\u66f4\u503e\u5411\u4e8e\u51fa\u73b0G\u78b1\u57fa，\u662fMotif\u7684\u6838\u5fc3\u4fdd\u5b88\u4f4d\u70b9\u3002<\/p>\n

（\u4e09）\u4e00\u81f4\u6027\u5e8f\u5217：\u7b80\u5316\u7684\u6838\u5fc3\u5e8f\u5217\u7279\u5f81<\/p>\n

\u4e00\u81f4\u6027\u5e8f\u5217\u662fMotif\u6838\u5fc3\u7279\u5f81\u7684\u7b80\u5316\u6587\u5b57\u8868\u5f81，\u5b83\u901a\u8fc7\u5355\u4e00\u5b57\u7b26\u4e32\u6982\u62ecMotif\u4e2d\u6bcf\u4e2a\u4f4d\u7f6e\u6700\u504f\u597d\u7684\u78b1\u57fa（\u6216\u5141\u8bb8\u7684\u78b1\u57fa\u7c7b\u578b），\u672c\u8d28\u662fLogo\u56fe\u7684\u201c\u6587\u5b57\u6d53\u7f29\u7248\u201d，\u4fbf\u4e8e\u5feb\u901f\u8bb0\u5f55\u548c\u4ea4\u6d41\u6838\u5fc3\u4fe1\u606f\u3002\u4e00\u81f4\u6027\u5e8f\u5217\u7684\u6700\u5927\u4f18\u52bf\u662f\u7b80\u6d01\u6613\u61c2，\u9002\u5408\u7528\u4e8e\u6587\u7ae0\u6458\u8981\u3001\u7ed3\u679c\u8868\u683c\u6216\u53e3\u5934\u6c47\u62a5\u4e2d\u5feb\u901f\u4f20\u9012Motif\u6838\u5fc3\u4fe1\u606f\u3002\u5728ChIP\/CUT\u7ed3\u679c\u89e3\u8bfb\u4e2d，\u5c06\u9884\u6d4b\u5f97\u5230\u7684\u4e00\u81f4\u6027\u5e8f\u5217\u4e0eJASPAR\u7b49\u6570\u636e\u5e93\u4e2d\u7684\u5df2\u77e5\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408Motif\u6bd4\u5bf9，\u53ef\u5feb\u901f\u521d\u6b65\u9501\u5b9a\u6f5c\u5728\u7684\u7ed3\u5408\u8f6c\u5f55\u56e0\u5b50\u3002\u9700\u8981\u6ce8\u610f\u7684\u662f，\u4e00\u81f4\u6027\u5e8f\u5217\u4f1a\u4e22\u5931\u78b1\u57fa\u9891\u7387\u7684\u5b9a\u91cf\u4fe1\u606f（\u4ec5\u4fdd\u7559\u201c\u504f\u597d\/\u5141\u8bb8\u201d\u7684\u5b9a\u6027\u4fe1\u606f），\u56e0\u6b64\u4e0d\u80fd\u66ff\u4ee3Logo\u56fe\u6216PFM\u77e9\u9635\u8fdb\u884c\u6df1\u5ea6\u5206\u6790\u3002<\/p>\n<\/p>\n

\u8868 IUPAC\u7b80\u5e76\u78b1\u57fa\u7b26\u53f7\u6807\u51c6\u5bf9\u5e94\u8868<\/p>\n<\/p>\n

\"image.png\"<\/p>\n

\u4e00\u81f4\u6027\u5e8f\u5217\u7684\u6784\u5efa\u89c4\u5219\u57fa\u4e8e\u78b1\u57fa\u9891\u7387：\u5bf9\u4e8e\u6bcf\u4e2a\u4f4d\u7f6e，\u4f18\u5148\u9009\u62e9\u51fa\u73b0\u9891\u7387\u6700\u9ad8\u7684\u78b1\u57fa\u4f5c\u4e3a\u6838\u5fc3\u78b1\u57fa；\u82e5\u591a\u4e2a\u78b1\u57fa\u51fa\u73b0\u9891\u7387\u76f8\u8fd1（\u65e0\u7edd\u5bf9\u4f18\u52bf），\u5219\u91c7\u7528IUPAC\u6807\u51c6\u6a21\u7cca\u78b1\u57fa\u4ee3\u7801\u8868\u793a\u3002\u4f8b\u5982，R\u4ee3\u8868A\u6216G（\u560c\u5464）\u3001Y\u4ee3\u8868C\u6216T（\u5627\u5576）\u3001N\u4ee3\u8868\u4efb\u610f\u78b1\u57fa\u7b49\u3002\u4ee5\u4e00\u81f4\u6027\u5e8f\u5217\u201cATRGCTY\u201d\u4e3a\u4f8b，\u5176\u542b\u4e49\u4e3a：\u7b2c1\u4f4d\u56fa\u5b9a\u4e3aA，\u7b2c2\u4f4d\u56fa\u5b9a\u4e3aT，\u7b2c3\u4f4d\u4e3aA\u6216G，\u7b2c4\u4f4d\u4e3aG，\u7b2c5\u4f4d\u4e3aC，\u7b2c6\u4f4d\u4e3aT，\u7b2c7\u4f4d\u4e3aC\u6216T\u3002<\/p>\n

（\u56db）PFM\/PWM\u77e9\u9635<\/p>\n

PFM\u77e9\u9635\u662fMotif\u6700\u539f\u59cb\u3001\u6700\u7cbe\u51c6\u7684\u5b9a\u91cf\u8868\u5f81\u5f62\u5f0f，\u76f4\u63a5\u8bb0\u5f55\u4e86Motif\u6bcf\u4e2a\u4f4d\u7f6e\u4e0a4\u79cd\u78b1\u57fa（A\u3001T\u3001C\u3001G）\u7684\u51fa\u73b0\u9891\u7387\u6216\u8ba1\u6570，\u662f\u6784\u5efaLogo\u56fe\u548c\u4e00\u81f4\u6027\u5e8f\u5217\u7684\u57fa\u7840\u6570\u636e\u6765\u6e90\u3002PFM\u77e9\u9635\u7684\u6838\u5fc3\u4ef7\u503c\u5728\u4e8e\u63d0\u4f9b\u7cbe\u51c6\u7684\u5b9a\u91cf\u6570\u636e，\u652f\u6491\u540e\u7eed\u7684\u6df1\u5ea6\u5206\u6790\u3002\u4f8b\u5982，\u5c06PFM\u77e9\u9635\u8f6c\u6362\u4e3aPWM\u77e9\u9635（\u4f4d\u7f6e\u6743\u91cd\u77e9\u9635）\u540e，\u53ef\u7528\u4e8e\u5168\u57fa\u56e0\u7ec4\u8303\u56f4\u5185\u7684Motif\u4f4d\u70b9\u626b\u63cf（\u5982FIMO\u5de5\u5177），\u7cbe\u51c6\u5b9a\u4f4d\u6f5c\u5728\u7684\u8f6c\u5f55\u56e0\u5b50\u7ed3\u5408\u4f4d\u70b9；\u901a\u8fc7\u5bf9\u6bd4\u4e0d\u540c\u6837\u672c\u7684PFM\u77e9\u9635，\u8fd8\u80fd\u91cf\u5316\u5206\u6790Motif\u78b1\u57fa\u504f\u597d\u6027\u7684\u7ec6\u5fae\u5dee\u5f02\u3002\u6b64\u5916，PFM\u77e9\u9635\u4e5f\u662fMotif\u6570\u636e\u5e93（\u5982JASPAR）\u5b58\u50a8\u6570\u636e\u7684\u6838\u5fc3\u683c\u5f0f，\u4fbf\u4e8e\u8de8\u5de5\u5177\u3001\u8de8\u7814\u7a76\u7684\u6570\u636e\u5206\u6790\u4e0e\u590d\u7528\u3002<\/p>\n<\/p>\n

\"\u56fe\u72473.png\"<\/p>\n

\u77e9\u9635\u5f62\u5f0f\u4e3a\u4e8c\u7ef4\u77e9\u9635，\u884c\u4ee3\u88684\u79cd\u78b1\u57fa（\u901a\u5e38\u6309A\u3001T\u3001C\u3001G\u6392\u5e8f），\u5217\u4ee3\u8868Motif\u7684\u5e8f\u5217\u4f4d\u7f6e（\u4ece5\u2019\u7aef\u52303\u2019\u7aef）\u3002\u77e9\u9635\u4e2d\u7684\u6bcf\u4e2a\u6570\u503c\u4ee3\u8868\u5bf9\u5e94\u78b1\u57fa\u5728\u8be5\u4f4d\u7f6e\u7684\u51fa\u73b0\u6b21\u6570（\u6216\u9891\u7387）\u3002<\/p>\n

\u2014\u2014\u6ce8：<\/p>\n

\u5feb\u901fMotif\u77e9\u9635\u56fe\u53ef\u89c6\u5316\u5c55\u793a\u7684\u65b9\u6cd5<\/p>\n

（1）\u5728\u7ed3\u9898\u62a5\u544aMotif\u5206\u6790\u8868\u683c\u4e2d\u627e\u5230\u76f8\u5e94\u7684motif\u5e8f\u5217，\u70b9\u51fb\u6700\u540e\u7684martix\u83b7\u5f97\u76f8\u5e94\u7684\u77e9\u9635\u4fe1\u606f；<\/p>\n

（2）https:\/\/logojs.wenglab.org\/app\/create\/ \u5728\u8fd9\u4e2a\u7f51\u7ad9\u4e0a\u7c98\u8d34\u4e0amotif\u77e9\u9635，\u751f\u6210\u56fe\u7247\u540e\u4fdd\u5b58\u57ceSVG\u7684\u683c\u5f0f\u3002<\/p>\n<\/p>\n

\u5c0f\u7ed3<\/h2>\n<\/p>\n

\u63d0\u4f9b\u4eceChIP\/CUT&Tag\u5b9e\u9a8c\u5230\u751f\u4fe1\u5206\u6790\u7684\u4e00\u7ad9\u5f0f\u670d\u52a1：\u2460\u5b9e\u9a8c\u5c42\u9762：\u91c7\u7528\u7a33\u5b9a\u4f18\u5316\u7684\u5b9e\u9a8c\u6d41\u7a0b，，\u652f\u6301\u5c11\u91cf\u7ec6\u80de\u548c\u4e34\u5e8a\u6837\u672c，\u786e\u4fdd\u6570\u636e\u7684\u9ad8\u4fe1\u566a\u6bd4；\u2461\u5206\u6790\u5c42\u9762：\u63d0\u4f9b\u5168\u9762\u7684\u751f\u4fe1\u5206\u6790\u670d\u52a1，\u5305\u62ec\u4e2a\u6027\u5316\u5206\u6790，\u914d\u5907\u4e13\u4e1a\u7684\u6280\u672f\u652f\u6301\u89e3\u8bfb\u7ed3\u679c；\u2462\u9a8c\u8bc1\u5c42\u9762：\u53ef\u914d\u5957EMSA\u3001\u53cc\u8367\u5149\u7d20\u9176\u548cRT-qPCR\u7b49\u5b9e\u9a8c，\u9a8c\u8bc1Motif\u4e0eTF\u7684\u7ed3\u5408\u7279\u5f02\u6027\u53ca\u8c03\u63a7\u529f\u80fd\u3002<\/p>\n

\u5982\u679c\u5bf9ChIP\/CUT\u5b9e\u9a8c\u6709\u76f8\u5173\u95ee\u9898，\u6b22\u8fce\u5728\u8bc4\u8bba\u533a\u7559\u8a00，\u6211\u4eec\u5c06\u4e3a\u4f60\u63d0\u4f9b\u4e13\u4e1a\u7684\u89e3\u7b54；\u4e5f\u53ef\u8054\u7cfb\u6211\u4eec\u7684\u6280\u672f\u652f\u6301\u56e2\u961f，\u83b7\u53d6\u4e2a\u6027\u5316\u7684ChIP\/CUT\u6570\u636e\u5206\u6790\u670d\u52a1\u3002\u79d1\u7814\u4e4b\u8def，\u6b65\u6b65\u4e3a\u8425\u3002\u5e0c\u671b\u4eca\u5929\u7684\u6587\u7ae0\u80fd\u5e2e\u4f60\u5403\u900fMotif\u5206\u6790，\u8ba9ChIP\/CUT&Tag\u7814\u7a76\u66f4\u9ad8\u6548\u3001\u66f4\u6df1\u5165！<\/p>\n","protected":false},"excerpt":{"rendered":"

ChIP-seq（\u67d3\u8272\u8d28\u514d\u75ab\u6c89\u6dc0\u6d4b\u5e8f）\u548c\u8fd1\u5e74\u6765\u5927\u706b\u7684CUT&Tag（\u9776\u5411\u526a\u5207\u4e0e\u6807\u8bb0\u6280\u672f）\u662f\u8868\u89c2\u9057\u4f20\u5b66\u7814\u7a76\u7684\u201c\u9ec4\u91d1\u5de5\u5177\u201d，\u6838\u5fc3\u4f5c\u7528\u662f\u7cbe\u51c6\u5b9a\u4f4d\u86cb\u767d\u8d28（\u5982\u8f6c\u5f55\u56e0\u5b50\u3001\u7ec4\u86cb\u767d\u4fee\u9970）\u5728\u57fa\u56e0\u7ec4\u4e0a\u7684\u7ed3\u5408\u4f4d\u70b9\u3002\u800c\u5728ChIP\/CUT\u7ed3\u679c\u89e3\u8bfb\u4e2d，Motif\u5206\u6790\u7edd\u5bf9\u662f\u7ed5\u4e0d\u5f00\u7684\u6838\u5fc3\u73af\u8282，\u5b83\u80fd\u5e2e\u4f60\u4ece\u6d77\u91cf\u7ed3\u5408\u4f4d\u70b9\u4e2d，\u6316\u6398\u51fa\u86cb\u767d\u8d28\u8bc6\u522b\u7ed3\u5408\u7684\u6838\u5fc3DNA\u5e8f\u5217\u57fa\u5e8f&#xff0<\/p>\n","protected":false},"author":2,"featured_media":62641,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[222,99,50,207,427,78,2051],"topic":[],"class_list":["post-62645","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-server","tag-eclipse","tag-java","tag-50","tag-207","tag-427","tag-78","tag-2051"],"yoast_head":"\nChIP\/CUT\u7ed3\u679c\u89e3\u8bfb\u4e4bMotif\u5206\u6790\uff0c\u5145\u5206\u5229\u7528\u7ec4\u5b66\u6570\u636e - 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