{"id":123115,"date":"2026-11-06T13:00:39","date_gmt":"2026-11-06T05:00:39","guid":{"rendered":"https:\/\/www.wsisp.com\/helps\/123115.html"},"modified":"2026-11-06T13:00:39","modified_gmt":"2026-11-06T05:00:39","slug":"sh-peg-iba%ef%bc%9a%e5%b7%af%e5%9f%ba%e6%8e%a5%e5%88%b0%e9%87%91%e8%a1%a8%e9%9d%a2%ef%bc%8c%e6%8a%8a%e5%90%b2%e5%93%9a%e4%b8%81%e9%85%b8%e7%a7%8d%e5%9c%a8%e6%9d%90%e6%96%99%e4%b8%8a","status":"publish","type":"post","link":"https:\/\/www.wsisp.com\/helps\/123115.html","title":{"rendered":"SH-PEG-IBA\uff1a\u5def\u57fa\u63a5\u5230\u91d1\u8868\u9762\uff0c\u628a\u5432\u54da\u4e01\u9178\u201c\u79cd\u201d\u5728\u6750\u6599\u4e0a"},"content":{"rendered":"

\/* MD \/ \u5bcc\u6587\u672c\u4e2d\u7684 .toc\uff08\u542b\u535a\u5ba2\u56ed\u642c\u5bb6\u7b49\u5d4c\u5957\u7ed3\u6784\uff09\uff1b.toc-box \u5728\u4fa7\u680f\uff0c\u4e0d\u53d7\u5f71\u54cd *\/
\n #content_views .toc,
\n \/* \u7f16\u8f91\u5668\u5e38\u5728\u76ee\u5f55\u524d\u540e\u63d2\u5165\u7a7a p\uff08:empty \u4ecd\u5360 20px\uff09\uff0c\u4e00\u5e76\u53bb\u6389\u907f\u514d\u9876\u7a7a\u9699 *\/
\n #content_views.markdown_views > p:empty:has(+ .toc),
\n #content_views.markdown_views > .toc + p:empty,
\n \/* \u5bcc\u6587\u672c\u65e7\u7248\u76ee\u5f55\u6807\u8bb0 *\/
\n #content_views.htmledit_views #main-toc,
\n #content_views.htmledit_views #hr-toc,
\n #content_views.htmledit_views p[id*=\\”-toc\\”] {
\n display: none !important;
\n }
\n \/* \u76ee\u5f55\u53bb\u6389\u540e\uff0c\u7d27\u8ddf\u7684\u9996\u4e2a\u6807\u9898\u4e0d\u518d\u591a\u51fa\u4e00\u5757\u4e0a\u8fb9\u8ddd *\/
\n #content_views.markdown_views > .toc + h1,
\n #content_views.markdown_views > .toc + h2,
\n #content_views.markdown_views > .toc + h3,
\n #content_views.markdown_views > .toc + h4,
\n #content_views.markdown_views > .toc + p:empty + h1,
\n #content_views.markdown_views > .toc + p:empty + h2,
\n #content_views.markdown_views > .toc + p:empty + h3,
\n #content_views.markdown_views > .toc + p:empty + h4 {
\n margin-top: 0 !important;
\n }<\/p>\n

\u524d\u51e0\u7bc7\u8bb2\u7684 DSPE-PEG-IBA \u9760\u758f\u6c34\u5c3e\u63d2\u8fdb\u8102\u819c，\u9002\u5408\u505a\u8f7d\u4f53；\u800c\u505a\u8868\u9762\u4fee\u9970\u7684\u4eba\u9700\u8981\u7684\u662f\u53e6\u4e00\u79cd\u951a\u2014\u2014\u80fd\u7262\u7262\u6293\u4f4f\u91d1\u7535\u6781\u3001\u91d1\u82af\u7247\u6216\u91d1\u7eb3\u7c73\u7c92\u7684\u90a3\u79cd\u3002SH-PEG-IBA \u5c31\u662f\u4e3a\u6b64\u8bbe\u8ba1\u7684，\u5b83\u7684\u4e2d\u6587\u540d\u5def\u57fa-\u805a\u4e59\u4e8c\u9187-\u5432\u54da\u4e01\u9178，\u4e00\u7aef\u662f\u5def\u57fa（-SH），\u4e2d\u95f4\u662f PEG \u94fe，\u672b\u7aef\u662f IBA\u3002\u5b83\u4e0d\u53c2\u4e0e\u81ea\u7ec4\u88c5\u6210\u56ca\u6ce1，\u4ef7\u503c\u5728\u4e8e\u628a\u529f\u80fd\u5c0f\u5206\u5b50\u7a33\u5b9a\u5730\u56fa\u5b9a\u5728\u56fa\u76f8\u8868\u9762\u3002\u4e0b\u9762\u4ece\u6784\u6210\u3001\u539f\u7406\u3001\u5e94\u7528\u51e0\u4e2a\u7ef4\u5ea6\u4ecb\u7ecd\u3002<\/p>\n

\u4e00\u3001\u5206\u5b50\u6784\u6210<\/p>\n

1.\u5def\u57fa\u7aef（-SH）：\u4e0e\u91d1\u3001\u94f6\u7b49\u8d35\u91d1\u5c5e\u8868\u9762\u5f62\u6210 Au\u2013S \u952e，\u4e5f\u53ef\u4e0e\u9a6c\u6765\u9170\u4e9a\u80fa\u3001\u4e59\u70ef\u57fa\u7b49\u57fa\u56e2\u53d1\u751f\u70b9\u51fb\/\u52a0\u6210\u53cd\u5e94，\u662f\u5206\u5b50\u7684\u201c\u951a\u70b9\u201d；
\n2.PEG \u6bb5：\u805a\u4e59\u4e8c\u9187\u94fe，\u5e38\u7528 2000\u30013400\u30015000 \u7b49\u89c4\u683c，\u628a\u529f\u80fd\u7aef\u4ece\u8868\u9762\u6491\u5f00，\u540c\u65f6\u964d\u4f4e\u975e\u7279\u5f02\u5438\u9644；
\n3.IBA \u6bb5：\u5432\u54da\u4e01\u9178，\u5432\u54da\u73af + \u4e01\u9178\u4fa7\u94fe，\u7fa7\u57fa\u4e3a\u5076\u8054\u4f4d\u70b9\u6216\u6e38\u79bb\u5c55\u793a\u4f4d\u70b9\u3002
\n\u4ea7\u54c1\u53c2\u6570\u53c2\u8003：\u767d\u8272\u81f3\u7c7b\u767d\u8272\u7c89\u672b，\u6216\u968f\u5206\u5b50\u91cf\u5448\u8721\u72b6\/\u7c98\u7a20\u72b6；\u6eb6\u4e8e\u6c34\u3001DMSO\u3001DMF\u3001\u7532\u9187；\u5efa\u8bae -20\u2103\u3001\u5e72\u71e5\u3001\u907f\u5149\u3001\u60f0\u6027\u6c14\u4f53\u4fdd\u62a4\u4fdd\u5b58\u2014\u2014\u5def\u57fa\u5728\u7a7a\u6c14\u4e2d\u4f1a\u7f13\u6162\u6c27\u5316\u6210\u4e8c\u786b\u952e\u3002
\n\"\"
\n\u4e8c\u3001\u4f5c\u7528\u539f\u7406<\/p>\n

1.Au\u2013S \u81ea\u7ec4\u88c5：\u5def\u57fa\u4e0e\u91d1\u8868\u9762\u4eb2\u548c\u529b\u5f3a，\u53ef\u5728\u91d1\u8868\u9762\u5f62\u6210\u81f4\u5bc6\u5355\u5206\u5b50\u5c42，\u628a PEG-IBA \u7a33\u5b9a\u56fa\u5b9a\u5728\u56fa\u76f8\u754c\u9762；
\n2.\u5def\u57fa-\u9a6c\u6765\u9170\u4e9a\u80fa\/\u5def\u57fa-\u70ef\u53cd\u5e94：\u5728\u6750\u6599\u5316\u5b66\u4e2d，\u5def\u57fa\u53ef\u4e0e\u9a6c\u6765\u9170\u4e9a\u80fa\u5728\u4e2d\u6027\u6761\u4ef6\u4e0b\u5feb\u901f\u6210\u952e，\u6216\u4e0e\u70ef\u70c3\u8fdb\u884c\u5149\u5f15\u53d1\u70b9\u51fb，\u7528\u4e8e\u6c34\u51dd\u80f6\u3001\u5fae\u7403\u8868\u9762\u63a5\u679d；
\n3.PEG \u7684\u95f4\u9694\u4e0e\u6297\u5438\u9644：\u94fe\u957f\u51b3\u5b9a\u529f\u80fd\u7aef\u79bb\u8868\u9762\u7684\u8ddd\u79bb，\u4e5f\u51b3\u5b9a\u8868\u9762\u5bf9\u86cb\u767d\u3001\u9897\u7c92\u7684\u975e\u7279\u5f02\u5438\u9644\u7a0b\u5ea6；
\n4.IBA \u7aef\u5c55\u793a：\u7fa7\u57fa\u6e38\u79bb\u65f6\u53ef\u7ee7\u7eed\u505a EDC\/NHS \u6d3b\u5316\u5076\u8054，\u4e5f\u53ef\u4fdd\u6301\u6e38\u79bb\u72b6\u6001\u7528\u4e8e\u7814\u7a76\u8868\u9762\u751f\u957f\u7d20\u4fe1\u53f7\u5bf9\u7ec6\u80de\u6216\u7ec4\u7ec7\u884c\u4e3a\u7684\u5f71\u54cd\u3002<\/p>\n

\u4e09\u3001\u5b9e\u9a8c\u5e94\u7528<\/p>\n

1.\u91d1\u8868\u9762\u529f\u80fd\u5316：\u91d1\u7535\u6781\u3001SPR\/QCM \u82af\u7247\u3001\u91d1\u7eb3\u7c73\u7c92\u8868\u9762\u4fee\u9970，\u6784\u5efa\u5e26\u751f\u957f\u7d20\u7c7b\u529f\u80fd\u7aef\u7684\u754c\u9762；
\n2.\u8868\u9762\u914d\u4f53\u5c55\u793a：\u5728\u82af\u7247\u6216\u9897\u7c92\u8868\u9762\u5448\u73b0 IBA，\u7814\u7a76\u754c\u9762\u4fe1\u53f7\u5bf9\u7ec6\u80de\u9ecf\u9644\u3001\u6781\u6027\u751f\u957f\u7b49\u884c\u4e3a\u7684\u5f71\u54cd；
\n3.\u6750\u6599\u63a5\u679d：\u4e0e\u9a6c\u6765\u9170\u4e9a\u80fa\u4fee\u9970\u7684\u805a\u5408\u7269\u3001\u6c34\u51dd\u80f6\u524d\u4f53\u53cd\u5e94，\u628a IBA \u5f15\u5165\u4e09\u7ef4\u7f51\u7edc；
\n4.\u7eb3\u7c73\u91d1\u7a33\u5b9a\u5316：PEG \u94fe\u63d0\u4f9b\u7a7a\u95f4\u4f4d\u963b，\u51cf\u5c11\u76d0\u8bf1\u5bfc\u805a\u96c6，\u540c\u65f6\u8d4b\u4e88\u529f\u80fd\u7aef；
\n5.\u5bf9\u7167\u8bbe\u8ba1：SH-PEG（\u65e0 IBA）\u5bf9\u7167\u533a\u5206 PEG \u5c42\u672c\u8eab\u7684\u6548\u5e94，\u6e38\u79bb IBA \u7ade\u4e89\u5bf9\u7167\u9a8c\u8bc1\u529f\u80fd\u7aef\u7279\u5f02\u6027，\u7a7a\u767d\u91d1\u7247\/\u88f8\u9897\u7c92\u5bf9\u7167\u63d0\u4f9b\u57fa\u7ebf\u3002<\/p>\n

\u56db\u3001\u5b9e\u9a8c\u6ce8\u610f<\/p>\n

\u261e\u5def\u57fa\u6c27\u5316\u662f\u5934\u53f7\u95ee\u9898：\u5f00\u74f6\u524d\u56de\u6e29\u81f3\u5ba4\u6e29\u518d\u5f00\u76d6，\u51cf\u5c11\u6c34\u6c7d\u4e0e\u6c27\u6c14\u8fdb\u5165；\u6eb6\u6db2\u5c3d\u91cf\u73b0\u914d\u73b0\u7528，\u5fc5\u8981\u65f6\u901a\u6c14\u9664\u6c27；\u82e5\u5df2\u90e8\u5206\u6c27\u5316，\u53ef\u7528 TCEP \u8fd8\u539f\u540e\u518d\u7528，\u4e0d\u8981\u7528 DTT\u2014\u2014DTT \u672c\u8eab\u542b\u6e38\u79bb\u5def\u57fa，\u4f1a\u4e0e\u76ee\u6807\u5206\u5b50\u7ade\u4e89\u91d1\u8868\u9762\u4f4d\u70b9；
\n\u261e\u8868\u9762\u5bc6\u5ea6\u9700\u8981\u201c\u7a00\u91ca\u6cd5\u201d\u63a7\u5236：\u5355\u4e00 SH-PEG-IBA \u5bc6\u96c6\u6392\u5217\u65f6，PEG \u94fe\u95f4\u4f4d\u963b\u4f1a\u8ba9\u529f\u80fd\u7aef\u96be\u4ee5\u63a5\u8fd1，\u5efa\u8bae\u4e0e\u77ed\u94fe PEG-SH \u6309\u6bd4\u4f8b\u6df7\u914d，\u517c\u987e\u5bc6\u5ea6\u4e0e\u53ef\u53ca\u6027；
\n\u261e\u91d1\u8868\u9762\u5148\u6e05\u6d17：\u91d1\u7247\/\u7535\u6781\u4f7f\u7528\u524d\u9700\u5f7b\u5e95\u53bb\u9664\u6709\u673a\u6b8b\u7559\u4e0e\u6c27\u5316\u5c42，\u5426\u5219\u6210\u819c\u4e0d\u5300\u3001\u4fe1\u53f7\u6f02\u79fb，\u8868\u9762\u7b49\u79bb\u5b50\u4f53\u5171\u632f\u7c7b\u5b9e\u9a8c\u5c24\u5176\u654f\u611f；
\n\u261e\u5432\u54da\u907f\u5149：IBA \u6bb5\u89c1\u5149\u6613\u964d\u89e3，\u4fee\u9970\u4e0e\u4fdd\u5b58\u5168\u7a0b\u907f\u5149；
\n\u261e\u7fa7\u57fa\u6d3b\u5316\u7684\u76f8\u5bb9\u6027：\u505a EDC\/NHS \u5076\u8054\u65f6\u907f\u514d\u4f53\u7cfb\u4e2d\u540c\u65f6\u5b58\u5728\u542b\u5def\u57fa\u8fd8\u539f\u5242，\u4ee5\u514d\u8fd8\u539f\u5242\u5e72\u6270\u6d3b\u5316\u4e2d\u95f4\u4f53；
\n\u261e\u6d53\u5ea6\u4e0e\u6807\u8bb0\u5ea6\u786e\u8ba4：PEG \u6bb5\u65e0\u7279\u5f81\u7d2b\u5916\u5438\u6536，\u53ef\u7528\u5432\u54da\u73af\u5728 280 nm \u9644\u8fd1\u7684\u5438\u6536\u7c97\u7565\u5b9a\u91cf IBA \u7aef，\u914d\u5408 NMR \u6216\u8d28\u8c31\u786e\u8ba4\u7ed3\u6784；
\n\u261e\u4fdd\u5b58\u4e0e\u53d6\u7528：\u51bb\u5e72\u7c89 -20\u2103 \u907f\u5149\u60f0\u6027\u6c14\u4f53\u4fdd\u5b58，\u5206\u88c5\u907f\u514d\u53cd\u590d\u51bb\u878d；\u6eb6\u6db2\u6001\u4e0d\u5efa\u8bae\u957f\u671f\u5b58\u653e\u3002<\/p>\n

SH-PEG-IBA \u89e3\u51b3\u7684\u662f\u201c\u600e\u4e48\u628a\u529f\u80fd\u5c0f\u5206\u5b50\u7a33\u7a33\u6302\u5728\u56fa\u4f53\u8868\u9762\u201d\u8fd9\u4e2a\u95ee\u9898：\u5def\u57fa\u7ba1\u6293\u3001PEG \u7ba1\u6491\u3001IBA \u7ba1\u7528\u3002\u505a\u754c\u9762\u5b9e\u9a8c\u65f6，\u628a\u5def\u57fa\u7684\u6297\u6c27\u5316\u3001\u8868\u9762\u7684\u5bc6\u5ea6\u63a7\u5236\u8fd9\u4e24\u4ef6\u4e8b\u5904\u7406\u597d，\u6570\u636e\u91cd\u590d\u6027\u624d\u6709\u4fdd\u969c\u3002<\/p>\n

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