{"id":102852,"date":"2026-09-09T15:57:42","date_gmt":"2026-09-09T07:57:42","guid":{"rendered":"https:\/\/www.wsisp.com\/helps\/102852.html"},"modified":"2026-09-09T15:57:42","modified_gmt":"2026-09-09T07:57:42","slug":"%e8%b0%b7%e8%83%b1%e7%94%98%e8%82%bd%e8%bf%98%e5%8e%9f%e9%85%b6%ef%bc%88gr%ef%bc%89%e5%be%ae%e9%87%8f%e6%b3%95%e6%b5%8b%e5%ae%9a%e5%ae%9e%e9%aa%8c%e5%85%a8%e6%b5%81%e7%a8%8b%e4%b8%8e%e5%85%b3%e9%94%ae","status":"publish","type":"post","link":"https:\/\/www.wsisp.com\/helps\/102852.html","title":{"rendered":"\u8c37\u80f1\u7518\u80bd\u8fd8\u539f\u9176\uff08GR\uff09\u5fae\u91cf\u6cd5\u6d4b\u5b9a\u5b9e\u9a8c\u5168\u6d41\u7a0b\u4e0e\u5173\u952e\u907f\u5751\u6307\u5357"},"content":{"rendered":"

\u5728\u7ec6\u80de\u62b5\u5fa1\u6c27\u5316\u5e94\u6fc0（Oxidative Stress）\u4e0e\u7ef4\u6301\u5185\u73af\u5883\u8fd8\u539f\u7a33\u6001\u7684\u7f51\u7edc\u4e2d，\u8c37\u80f1\u7518\u80bd\u8fd8\u539f\u9176（Glutathione Reductase, \u7b80\u79f0 GR; EC 1.8.1.7） \u5360\u636e\u7740\u65e0\u53ef\u66ff\u4ee3\u7684\u201c\u5f15\u64ce\u201d\u5730\u4f4d\u3002GR \u662f\u4e00\u7c7b\u4f9d\u8d56\u4e8c\u6838\u82f7\u9178（FAD）\u7684\u9ec4\u7d20\u4e8c\u805a\u4f53\u6c27\u5316\u8fd8\u539f\u9176，\u50ac\u5316\u6c27\u5316\u578b\u8c37\u80f1\u7518\u80bd（GSSG）\u8fd8\u539f\u4e3a\u8fd8\u539f\u578b\u8c37\u80f1\u7518\u80bd（GSH），\u540c\u65f6\u6d88\u8017\u4e00\u5206\u5b50\u8fd8\u539f\u578b\u8f85\u9176 II（NADPH）\u3002<\/p>\n

\u7ec6\u80de\u5185\u7684\u8fd8\u539f\u578b\u8c37\u80f1\u7518\u80bd（GSH）\u662f\u6e05\u9664\u6d3b\u6027\u6c27（ROS）\u3001\u8fc7\u6c27\u5316\u6c22（H2O2）\u53ca\u91cd\u91d1\u5c5e\u6bd2\u7d20\u7684\u4e3b\u8981\u975e\u9176\u4fc3\u6297\u6c27\u5316\u5242\u3002\u5f53\u7ec6\u80de\u53d7\u5230\u6c27\u5316\u635f\u4f24\u65f6，GSH \u88ab\u8c37\u80f1\u7518\u80bd\u8fc7\u6c27\u5316\u7269\u9176（GSH-Px）\u8f6c\u5316\u4e3a GSSG；\u800c GR \u5219\u662f\u5c06 GSSG\u201c\u56de\u6536\u201d\u5e76\u91cd\u65b0\u8f6c\u5316\u4e3a GSH \u7684\u552f\u4e00\u5173\u952e\u9176\u3002\u56e0\u6b64，GR \u6d3b\u6027\u76f4\u63a5\u51b3\u5b9a\u4e86\u7ec6\u80de\u5185 GSH\/GSSG \u6bd4\u4f8b（\u7ec6\u80de\u6c27\u5316\u8fd8\u539f\u72b6\u6001\u7684\u9ec4\u91d1\u6307\u6807） \u7684\u9ad8\u4f4e\u3002\u57fa\u4e8e Abbkine\u4e9a\u79d1\u56e0 \u6807\u51c6\u5316\u5b9e\u9a8c\u89c4\u7a0b，\u672c\u6587\u7cfb\u7edf\u68b3\u7406\u4e86 CheKine\u2122 \u8c37\u80f1\u7518\u80bd\u8fd8\u539f\u9176（GR）\u6d3b\u6027\u68c0\u6d4b\u8bd5\u5242\u76d2（\u5fae\u91cf\u6cd5）\u7684\u5b9e\u9a8c\u5168\u6d41\u7a0b\u3001\u6837\u672c\u51c6\u5907\u89c4\u8303\u53ca\u5173\u952e\u907f\u5751\u6307\u5357\u3002<\/p>\n

\u4e00\u3001 \u68c0\u6d4b\u539f\u7406\u4e0e NADPH \u6d88\u8017\u8fde\u7eed\u5149\u8c31\u6cd5<\/h4>\n

\u5fae\u91cf\u6cd5\u6d4b\u5b9a GR \u6d3b\u6027\u57fa\u4e8e GSSG \u8fd8\u539f\u8fc7\u7a0b\u4e2d\u6d88\u8017 NADPH \u7684\u7d2b\u5916\u5438\u6536\u901f\u7387\u76d1\u6d4b：<\/p>\n

  • \u9176\u4fc3\u8fd8\u539f\u53cd\u5e94：\u5728\u53cd\u5e94\u4f53\u7cfb\u4e2d\u52a0\u5165\u5e95\u7269 GSSG \u548c\u7535\u5b50\u4f9b\u4f53 NADPH\u3002\u6837\u672c\u4e2d\u7684 GR \u50ac\u5316 GSSG \u4e0e NADPH \u53cd\u5e94，\u751f\u6210\u4e24\u5206\u5b50 GSH \u548c\u4e00\u5206\u5b50 NADP+\u3002<\/li>\n
  • 340 nm \u7d2b\u5916\u52a8\u6001\u76d1\u6d4b：NADPH \u5728 340 nm \u6ce2\u957f \u5904\u5177\u6709\u5f3a\u70c8\u7684\u7279\u5f81\u7d2b\u5916\u5149\u5438\u6536\u5cf0，\u800c\u6c27\u5316\u578b NADP+ \u5728\u8be5\u6ce2\u957f\u5904\u65e0\u5438\u6536\u3002\u5728\u8fde\u7eed\u53cd\u5e94\u8fc7\u7a0b\u4e2d，340 nm \u5904\u7684\u5438\u5149\u5ea6\u4e0b\u964d\u901f\u7387（\u0394A\/min）\u4e0e GR \u7684\u50ac\u5316\u6d3b\u6027\u5448\u4e25\u5bc6\u7684\u7ebf\u6027\u6b63\u6bd4\u5173\u7cfb\u3002<\/li>\n
  • \u6d3b\u6027\u5b9a\u91cf\u8ba1\u7b97：\u6839\u636e NADPH \u7684\u6469\u5c14\u6d88\u5149\u7cfb\u6570，\u8ba1\u7b97\u51fa\u5355\u4f4d\u8d28\u91cf\u7ec4\u7ec7\u3001\u7ec6\u80de\u6216\u86cb\u767d\u8d28\u5728\u5355\u4f4d\u65f6\u95f4\u5185\u6d88\u8017 NADPH \u7684\u5fae\u6469\u5c14\u6570（umol\/min\/mg protein \u6216 U\/mg protein）\u3002<\/li>\n

    \u4e8c\u3001 \u6837\u672c\u91c7\u96c6\u3001\u524d\u5904\u7406\u4e0e\u4fdd\u5b58\u89c4\u8303<\/h4>\n
      \n
    • \u52a8\u690d\u7269\u7ec4\u7ec7\u4e0e\u7ec6\u80de\u6837\u672c：\u7cbe\u786e\u79f0\u53d6\u65b0\u9c9c\u7ec4\u7ec7（\u5982\u809d\u810f\u3001\u7ea2\u7ec6\u80de\u3001\u690d\u7269\u53f6\u7247\u7ea6 0.1 g），\u52a0\u5165\u9884\u51b7\u7684\u63d0\u53d6\u6db2\u3002\u7f6e\u4e8e\u51b0\u6c34\u6d74\u4e2d\u8fdb\u884c\u5300\u6d46\u6216\u8d85\u58f0\u7834\u788e（\u907f\u514d\u8fc7\u70ed\u5bfc\u81f4 GR \u86cb\u767d\u53d8\u6027\u5931\u6d3b）\u30024\u2103\u300110,000 g \u79bb\u5fc3 10 \u5206\u949f，\u5c0f\u5fc3\u5438\u53d6\u4e0a\u6e05\u6db2，\u7f6e\u4e8e\u51b0\u4e0a\u5f85\u6d4b\u3002<\/li>\n
    • \u8840\u6e05\u4e0e\u7ea2\u7ec6\u80de\u6837\u672c： \u5168\u8840\u79bb\u5fc3\u5206\u79bb\u7ea2\u7ec6\u80de，\u7528\u9884\u51b7\u751f\u7406\u76d0\u6c34\u6d17\u6da4\u540e，\u52a0\u5165\u51b7\u84b8\u998f\u6c34\u88c2\u89e3\u7ea2\u7ec6\u80de，\u79bb\u5fc3\u53d6\u4e0a\u6e05\u6d4b\u5b9a（\u7ea2\u7ec6\u80de\u4e2d\u542b\u6709\u6781\u9ad8\u6d3b\u6027\u7684 GR）\u3002<\/li>\n
    • \u4fdd\u5b58\u89c4\u8303： \u7c97\u63d0\u6db2\u4e2d\u7684 GR \u9176\u6d3b\u76f8\u5bf9\u7a33\u5b9a，\u4f46\u63d0\u53d6\u540e\u4ecd\u5efa\u8bae\u7f6e\u4e8e\u51b0\u4e0a\u5e76\u5728\u5f53\u5929\u7684 4-6 \u5c0f\u65f6\u5185\u5b8c\u6210\u4e0b\u673a\u6d4b\u5b9a；\u82e5\u9700\u957f\u671f\u4fdd\u5b58，\u53ef\u5728 -80\u2103 \u51bb\u5b58，\u4f46\u5e94\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002<\/li>\n<\/ul>\n

      \u4e09\u3001 \u5fae\u91cf\u6cd5\u52a0\u6837\u4e0e\u6bd4\u8272\u6d4b\u5b9a\u6b65\u9aa4<\/h4>\n

      \u672c\u8bd5\u5242\u76d2\u9002\u914d \u7d2b\u5916\u900f\u5149 96 \u5b54\u677f（UV Plate） \u6216\u77f3\u82f1\u6bd4\u8272\u76bf，\u91c7\u7528\u8fde\u7eed\u52a8\u529b\u5b66\u6bd4\u8272\u6cd5：<\/p>\n

    • \u4eea\u5668\u9884\u70ed\u4e0e\u6ce2\u957f\u8bbe\u5b9a：\u5f00\u542f\u9176\u6807\u4eea\u5e76\u9884\u70ed，\u8bbe\u5b9a\u6d4b\u5b9a\u6ce2\u957f\u4e3a 340 nm；\u5b75\u80b2\u6a21\u5757\u8bbe\u5b9a\u4e3a 37\u2103（\u52a8\u7269\/\u4eba\u4f53\u6837\u672c）\u6216 25\u2103\/30\u2103（\u690d\u7269\/\u5fae\u751f\u7269\u6837\u672c）\u3002<\/li>\n
    • \u8bd5\u5242\u5de5\u4f5c\u6db2\u914d\u5236：\u5c06\u8bd5\u5242\u76d2\u4e2d\u7684\u5e95\u7269\u7f13\u51b2\u6db2（\u542b GSSG）\u53ca NADPH \u6db2\u6309\u8bf4\u660e\u4e66\u6bd4\u4f8b\u590d\u914d，\u7f6e\u4e8e\u6307\u5b9a\u6e29\u5ea6\u4e0b\u9884\u70ed 10 \u5206\u949f\u3002<\/li>\n
    • \u5fae\u5b54\u677f\u52a0\u6837\u4e0e\u53cd\u5e94\u542f\u52a8：\u5411 96 \u5b54\u7d2b\u5916\u677f\u5404\u5b54\u4e2d\u52a0\u5165\u9002\u91cf\u7c97\u63d0\u6db2\u4e0a\u6e05\u3002\u5feb\u901f\u52a0\u5165\u9884\u70ed\u7684\u6df7\u5408\u53cd\u5e94\u5de5\u4f5c\u6db2，\u7acb\u5373\u6df7\u5300\u5e76\u653e\u5165\u9176\u6807\u4eea\u4e2d\u3002<\/li>\n
    • \u8fde\u7eed\u52a8\u529b\u5b66\u8bfb\u6570：\u7acb\u5373\u8bfb\u53d6 340 nm \u5904\u7684\u521d\u59cb\u5438\u5149\u5ea6 A1（\u7b2c 20 \u79d2），\u968f\u540e\u5728\u6307\u5b9a\u6e29\u5ea6\u4e0b\u8fde\u7eed\u8bfb\u53d6，\u8bb0\u5f55\u7b2c 3 \u5206\u949f（\u6216 5 \u5206\u949f）\u65f6\u7684\u5438\u5149\u5ea6 A2\u3002\u8ba1\u7b97 \u0394A = A1 – A2 \u4ee5\u53ca\u6bcf\u5206\u949f\u5438\u5149\u5ea6\u53d8\u5316\u901f\u7387 \u0394A\/min，\u5bf9\u7167\u8ba1\u7b97\u516c\u5f0f\u6c42\u51fa GR \u9176\u6d3b\u6027\u3002<\/li>\n

      \u56db\u3001 \u5173\u952e\u907f\u5751\u6307\u5357\u4e0e\u5e38\u89c1\u95ee\u9898\u6392\u67e5<\/h4>\n
        \n
      • \u907f\u5751\u70b9\u4e00：\u8bef\u7528\u666e\u901a PS \u6750\u8d28\u5fae\u5b54\u677f\u5bfc\u81f4 340 nm \u4fe1\u53f7\u963b\u65ad \u666e\u901a\u805a\u82ef\u4e59\u70ef（PS）\u5fae\u5b54\u677f\u5bf9 340 nm \u8fd1\u7d2b\u5916\u5149\u6709\u6781\u5f3a\u7684\u5149\u5438\u6536（\u521d\u503c\u901a\u5e38 > 3.0）\u3002\u5fc5\u987b\u4f7f\u7528\u4e13\u7528\u6297\u7d2b\u5916 96 \u5b54\u677f（UV-transparent Plate），\u5426\u5219\u4eea\u5668\u65e0\u6cd5\u8bfb\u53d6\u6709\u6548\u6570\u636e\u3002<\/li>\n
      • \u907f\u5751\u70b9\u4e8c：NADPH \u8bd5\u5242\u53cd\u590d\u51bb\u878d\u53d8\u8d28\u5931\u6d3b NADPH \u6781\u6613\u5438\u6f6e\u4e14\u5728\u9178\u6027\u6216\u5149\u7167\u6761\u4ef6\u4e0b\u81ea\u53d1\u5206\u89e3\u3002NADPH \u6bcd\u6db2\u5e94\u907f\u5149\u5c0f\u91cf\u5206\u88c5\u51bb\u5b58，\u907f\u514d\u53cd\u590d\u51bb\u878d\u5bfc\u81f4\u672c\u5e95\u5438\u5149\u5ea6\u8fc7\u4f4e，\u5f71\u54cd\u53cd\u5e94\u7ebf\u6027\u8303\u56f4\u3002<\/li>\n
      • \u907f\u5751\u70b9\u4e09：\u5185\u6e90\u6027 NADPH \u6c27\u5316\u9176\u9ad8\u80cc\u666f\u5e72\u9884 \u67d0\u4e9b\u4ee3\u8c22\u65fa\u76db\u7684\u7ec4\u7ec7\u6837\u672c\u542b\u6709\u8f83\u9ad8\u7684\u5185\u6e90\u6027 NADPH \u6c27\u5316\u9176\u3002\u82e5\u53d1\u73b0\u4e0d\u52a0\u5e95\u7269 GSSG \u65f6 340 nm \u5438\u5149\u5ea6\u4f9d\u7136\u5feb\u901f\u4e0b\u964d，\u5fc5\u987b\u8bbe\u7f6e\u201c\u4e0d\u52a0 GSSG\u201d\u7684\u6837\u672c\u5bf9\u7167\u5b54\u8fdb\u884c\u80cc\u666f\u6263\u9664\u3002<\/li>\n
      • \u907f\u5751\u70b9\u56db：\u52a0\u6837\u6df7\u5300\u4ea7\u751f\u6c14\u6ce1 340 nm \u7d2b\u5916\u8bfb\u53d6\u5bf9\u6c14\u6ce1\u6781\u5ea6\u654f\u611f，\u6c14\u6ce1\u4f1a\u5bfc\u81f4\u5149\u7ebf\u6298\u5c04\u9020\u6210\u8bfb\u6570\u5267\u70c8\u6ce2\u52a8\u3002\u52a0\u6837\u540e\u5982\u53d1\u73b0\u6c14\u6ce1，\u53ef\u7528\u5fae\u91cf\u9488\u5934\u70b9\u7834\u540e\u518d\u4e0b\u673a\u68c0\u6d4b\u3002<\/li>\n<\/ul>\n

        \u4e94\u3001 \u5b9e\u9a8c\u5ba4\u5b89\u5168\u4e0e\u5e9f\u5f03\u7269\u5904\u7406<\/h4>\n

        \u672c\u8bd5\u5242\u76d2\u5404\u7ec4\u5206\u4e0d\u542b\u5f3a\u8150\u8680\u6027\u6216\u9ad8\u6bd2\u6027\u5316\u5b66\u54c1，\u4f46\u6d89\u53ca\u7d2b\u5916\u5149\u68c0\u6d4b\u4e0e\u9176\u5b66\u53cd\u5e94，\u64cd\u4f5c\u65f6\u8bf7\u7a7f\u6234\u6807\u51c6\u5b9e\u9a8c\u670d\u4e0e\u9632\u5316\u624b\u5957\u3002\u5b9e\u9a8c\u4ea7\u751f\u7684\u79bb\u5fc3\u7ba1\u4e0e\u53cd\u5e94\u5e9f\u6db2\u6309\u5e38\u89c4\u751f\u7269\u5316\u5b66\u5e9f\u5f03\u7269\u5206\u7c7b\u6536\u96c6\u5904\u7f6e\u3002<\/p>\n

        \u51c6\u786e\u6d4b\u5b9a\u8c37\u80f1\u7518\u80bd\u8fd8\u539f\u9176\u6d3b\u6027\u5bf9\u4e8e\u63ed\u793a\u7ec6\u80de\u6297\u6c27\u5316\u9632\u5fa1\u673a\u5236\u3001\u8bc4\u4f30\u6c27\u5316\u5e94\u6fc0\u635f\u4f24\u53ca\u836f\u7269\u6bd2\u7406\u5177\u6709\u91cd\u8981\u6307\u5bfc\u4ef7\u503c\u3002\u4f9d\u6258 Abbkine\u4e9a\u79d1\u56e0 \u6807\u51c6\u5316\u7684\u5fae\u91cf\u7d2b\u5916\u8fde\u7eed\u68c0\u6d4b\u4f53\u7cfb，\u79d1\u7814\u4eba\u5458\u80fd\u591f\u9ad8\u6548\u514b\u670d\u80cc\u666f\u5e72\u6270，\u83b7\u53d6\u9ad8\u91cd\u590d\u6027\u7684\u7cbe\u7ec6\u52a8\u529b\u5b66\u6570\u636e\u3002<\/p><\/p>\n","protected":false},"excerpt":{"rendered":"

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